ArticleBiophysical journal2008
Macromolecular crowding and size effects on probe microviscosity.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 115 citations in OpenAlex.
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- Comparative analysis of different macromolecular crowding agents in human tenocyte cultures.Biomaterials and biosystems · 2026Article
- Cellular mechanical properties in response to environmental viscosity imaged by Brillouin Microscopy.Communications biology · 2025Article
- Synthesis of Carboxymethyl Dextran-Coated Gold Nanoparticles as Stable and Storable Optical Labels for Ultrasensitive Plasmonic Nanoparticle-Linked Sorbent Assay.Sensors (Basel, Switzerland) · 2025Article
- Lipid raft proteomics identify endothelial myosin-9 (MYH9) as a regulator of low-density lipoprotein transcytosis and atherosclerosis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Molecular transport through nano-sized multipores of lipid vesicles: a COMSOL simulation study.European biophysics journal : EBJ · 2025Article
- Physical effects of crowdant size and concentration on collective microtubule polymerization.Biophysical journal · 2025Article
- Speckle fluctuations reveal dynamics of microparticles in fibrin scaffolds in a model of bacterial infection.npj biological physics and mechanics · 2025Article
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- ISWI catalyzes nucleosome sliding in condensed nucleosome arrays.bioRxiv : the preprint server for biology · 2023Article
- Dependence of diffusion ineLife · 2022Article
- Macromolecular crowding effects on the kinetics of opposing reactions catalyzed by alcohol dehydrogenase.Biochemistry and biophysics reports · 2021Article
- Antenna Protein Clustering In Vitro Unveiled by Fluorescence Correlation Spectroscopy.International journal of molecular sciences · 2021Article
- Crowding tunes the organization and mechanics of actin bundles formed by crosslinking proteins.FEBS letters · 2021Article
- Alteration of Protein Binding Affinities by Aqueous Two-Phase Systems Revealed by Pressure Perturbation.Scientific reports · 2020Article
- Cytoplasmic Parvovirus Capsids Recruit Importin Beta for Nuclear Delivery.Journal of virology · 2020Article
- Study of molecular transport through a single nanopore in the membrane of a giant unilamellar vesicle using COMSOL simulation.European biophysics journal : EBJ · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Development of biologically relevant crowding solutions necessitates improved understanding of how the relative size and density of mobile obstacles affect probe diffusion. Both the crowding density and relative size of each co-solute in a mixture will contribute to the measured microviscosity as assessed by altered translational mobility. Using multiphoton fluorescent correlation spectroscopy, this study addresses how excluded volume of dextran polymers from 10 to 500 kDa affect microviscosity quantified by measurements of calmodulin labeled with green fluorescent protein as the diffusing probe. Autocorrelation functions were fit using both a multiple-component model with maximum entropy method (MEMFCS) and an anomalous model. Anomalous diffusion was not detected, but fits of the data with the multiple-component model revealed separable modes of diffusion. When the dominant mode of diffusion from the MEMFCS analysis was used, we observed that increased excluded volume slows probe mobility as a simple exponential with crowder concentration. This behavior can be modeled with a single parameter, beta, which depends on the dextran size composition. Two additional modes of diffusion were observed using MEMFCS and were interpreted as unique microviscosities. The fast mode corresponded to unhindered free diffusion as in buffer, whereas the slower agreed well with the bulk viscosity. At 10% crowder concentration, one finds a microviscosity approximately three times that of water, which mimics that reported for intracellular viscosity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.