ArticleBiophysical journal2008
Combining fluorescence lifetime and polarization microscopy to discriminate phase separated domains in giant unilamellar vesicles.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 47 citations in OpenAlex.
- Partitioning of a Hybrid Lipid in Domains of Saturated and Unsaturated Lipids in a Model Cellular Membrane.ACS omega · 2021Article
- Article
- Molecular organization, localization and orientation of antifungal antibiotic amphotericin B in a single lipid bilayer.Scientific reports · 2016Article
- Single Molecule 3D Orientation in Time and Space: A 6D Dynamic Study on Fluorescently Labeled Lipid Membranes.Journal of fluorescence · 2016Article
- Ultimate use of two-photon fluorescence microscopy to map orientational behavior of fluorophores.Biophysical journal · 2014Article
- Probing orientational behavior of MHC class I protein and lipid probes in cell membranes by fluorescence polarization-resolved imaging.Biophysical journal · 2011Article
- Phase separation in lipid membranes.Cold Spring Harbor perspectives in biology · 2011Review
- Self-consistent mean-field model for palmitoyloleoylphosphatidylcholine-palmitoyl sphingomyelin-cholesterol lipid bilayers.Physical review. E, Statistical, nonlinear, and soft matter physics · 2011Article
- Development of fluorophore dynamics imaging as a probe for lipid domains in model vesicles and cell membranes.European biophysics journal : EBJ · 2011Article
- Peptide-induced domain formation in supported lipid bilayers: direct evidence by combined atomic force and polarized total internal reflection fluorescence microscopy.Biophysical journal · 2010Article
- Lateral organization of complex lipid mixtures from multiscale modeling.The Journal of chemical physics · 2010Article
- Quantitative imaging of molecular order in lipid membranes using two-photon fluorescence polarimetry.Biophysical journal · 2009Article
- Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using fluorescence lifetime microscopy we study the structure of lipid domains in giant unilamellar vesicles made from sphingomyelin, 1,2-dioleoyl-sn-glycero-3-phosphocholine, and cholesterol. Lifetimes and orientation of a derivative of the fluorescent probe DPH embedded in the membrane were measured for binary and ternary lipid mixtures incorporating up to 42 mol % of cholesterol. The results show that adding cholesterol always increases the lifetime of the probe studied. In addition, the analysis of the probe orientation indicates that cholesterol has little influence on the ordering of the sphingomyelin alkyl chains whereas it has a noticeable effect on the structure of the 1,2-dioleoyl-sn-glycero-3-phosphocholine chains. The measurements made on the orientation and lifetime of the probe show the structure of the membrane in its liquid ordered and liquid disordered domains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.