ArticleNucleic acids research2008
Dicistronic tRNA-5S rRNA genes in Yarrowia lipolytica: an alternative TFIIIA-independent way for expression of 5S rRNA genes.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
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- Genomic and transcriptomic insights into Trichomonascus vanleenenianus, a xylan-degrading yeast isolated from saproxylic insect larvae.BMC genomics · 2026Article
- Optimizing the CRISPR/Cas9 system for gene editing inEngineering microbiology · 2025Article
- YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering inMetabolic engineering communications · 2017Article
- Sequence Assembly of Yarrowia lipolytica Strain W29/CLIB89 Shows Transposable Element Diversity.PloS one · 2016Article
- Genetic characterization of Plectorhinchus mediterraneus yields important clues about genome organization and evolution of multigene families.BMC genetics · 2012Article
- Article
- Widespread occurrence of non-canonical transcription termination by human RNA polymerase III.Nucleic acids research · 2011Article
- Evolutionary dynamics of the 5S rDNA gene family in the mussel Mytilus: mixed effects of birth-and-death and concerted evolution.Journal of molecular evolution · 2010Article
- Review
- Detection and analysis of alternative splicing in Yarrowia lipolytica reveal structural constraints facilitating nonsense-mediated decay of intron-retaining transcripts.Genome biology · 2010Article
- Comparative genomics of protoploid Saccharomycetaceae.Genome research · 2009Article
- Biotransformation of acetophenone and its halogen derivatives byAnnals of microbiologyArticle
- Eukaryotic 5S rRNA biogenesis.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In eukaryotes, genes transcribed by RNA polymerase III (Pol III) carry their own internal promoters and as such, are transcribed as individual units. Indeed, a very few cases of dicistronic Pol III genes are yet known. In contrast to other hemiascomycetes, 5S rRNA genes of Yarrowia lipolytica are not embedded into the tandemly repeated rDNA units, but appear scattered throughout the genome. We report here an unprecedented genomic organization: 48 over the 108 copies of the 5S rRNA genes are located 3' of tRNA genes. We show that these peculiar tRNA-5S rRNA dicistronic genes are expressed in vitro and in vivo as Pol III transcriptional fusions without the need of the 5S rRNA gene-specific factor TFIIIA, the deletion of which displays a viable phenotype. We also report the existence of a novel putative non-coding Pol III RNA of unknown function about 70 nucleotide-long (RUF70), the 13 genes of which are devoid of internal Pol III promoters and located 3' of the 13 copies of the tDNA-Trp (CCA). All genes embedded in the various dicistronic genes, fused 5S rRNA genes, RUF70 genes and their leader tRNA genes appear to be efficiently transcribed and their products correctly processed in vivo.
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