ArticleAcademic radiology2008
Magnetic resonance imaging detects differences in migration between primary and immortalized neural stem cells.
Article in Academic radiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Superparamagnetic Iron Oxide Nanoparticles: Cytotoxicity, Metabolism, and Cellular Behavior in Biomedicine Applications.International journal of nanomedicine · 2021Review
- Grafted Neural Stem Cells Show Lesion-Specific Migration in Radiation-Injured Rat Brains.RSC advances · 2018Article
- Rac1 Guides Porf-2 to Wnt Pathway to Mediate Neural Stem Cell Proliferation.Frontiers in molecular neuroscience · 2017Article
- Fetal brain mTOR signaling activation in tuberous sclerosis complex.Cerebral cortex (New York, N.Y. : 1991) · 2014Article
- High-resolution magnetic resonance microscopy and diffusion tensor imaging to assess brain structural abnormalities in the murine mucopolysaccharidosis VII model.Journal of neuropathology and experimental neurology · 2014Article
- Engraftment of nonintegrating neural stem cells differentially perturbs cortical activity in a dose-dependent manner.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Transplantation of CD15-enriched murine neural stem cells increases total engraftment and shifts differentiation toward the oligodendrocyte lineage.Stem cells translational medicine · 2013Article
- Rapamycin prevents seizures after depletion of STRADA in a rare neurodevelopmental disorder.Science translational medicine · 2013Article
- Current challenges for the advancement of neural stem cell biology and transplantation research.Stem cell reviews and reports · 2012Review
- Cell tracking in cardiac repair: what to image and how to image.European radiology · 2012Review
- In vivo and ex vivo MR imaging of slowly cycling melanoma cells.Magnetic resonance in medicine · 2011Article
- The use of cellular magnetic resonance imaging to track the fate of iron-labeled multipotent stromal cells after direct transplantation in a mouse model of spinal cord injury.Molecular imaging and biology · 2011Article
- STRADalpha deficiency results in aberrant mTORC1 signaling during corticogenesis in humans and mice.The Journal of clinical investigation · 2010Article
- Gene expression profiling reveals early cellular responses to intracellular magnetic labeling with superparamagnetic iron oxide nanoparticles.Magnetic resonance in medicine · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
RATIONALE AND
objectivesThe study was performed to evaluate the effect of magnetic resonance imaging (MRI) contrast agent (super paramagnetic iron oxide [SPIO]) on differentiation and migration of primary murine neural stem cells (NSCs) in comparison to a neural stem cell line (C17.2). Because detection of labeled cells depends on the concentration of SPIO particles per imaging voxel, the study was performed at various concentrations of SPIO particles to determine the concentration that could be used for in vivo detection of small clusters of grafted cells. MATERIALS AND
methodsMurine primary NSCs or C17.2 cells were labeled with different concentrations of SPIO particles (0, 25, 100, and 250 microg Fe/mL) and in vitro assays were performed to assess cell differentiation. In vivo MRI was performed 7 weeks after neonatal transplantation of labeled cells to evaluate the difference in migration capability of the two cell populations.
resultsBoth the primary NSCs and the C17.2 cells differentiated to similar number of neurons (Map2ab-positive cells). Similar patterns of engraftment of C17.2 cells were seen in transplanted mice regardless of the SPIO concentration used. In vivo MRI detection of grafted primary and C17.2 cells was only possible when cells were incubated with 100 microg/mL or higher concentration of SPIO. Extensive migration of C17.2 cells throughout the brain was observed, whereas the migration of the primary NSCs was more restricted.
conclusionsEngraftment of primary NSCs can be detected noninvasively by in vivo MRI, and the presence of SPIO particles do not affect the viability, differentiation, or engraftment pattern of the donor cells.
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