ArticleCell and tissue research1991
Chicken cardiac myofibrillogenesis studied with antibodies specific for titin and the muscle and nonmuscle isoforms of actin and tropomyosin.
Article in Cell and tissue research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 72 citations in OpenAlex.
- Enhancing the Cardiogenic Potential of Human Mesenchymal Stem Cells via Extracellular Matrix Proteins.International journal of molecular and cellular medicine · 2024Article
- Tropomyosin 3 (TPM3) function in skeletal muscle and in myopathy.Skeletal muscle · 2023Review
- Extracellular Matrix in Regulation of Contractile System in Cardiomyocytes.International journal of molecular sciences · 2019Review
- Article
- Scaffolds and chaperones in myofibril assembly: putting the striations in striated muscle.Biophysical reviews · 2011Article
- Myofibril assembly visualized by imaging N-RAP, alpha-actinin, and actin in living cardiomyocytes.Experimental cell research · 2009Article
- Expression and alternative splicing of N-RAP during mouse skeletal muscle development.Cell motility and the cytoskeleton · 2008Article
- Role of nonmuscle myosin IIB and N-RAP in cell spreading and myofibril assembly in primary mouse cardiomyocytes.Cell motility and the cytoskeleton · 2008Article
- Cardiomyocyte cytoskeleton and myofibrillogenesis in healthy and diseased heart.Heart failure reviews · 2000Review
- Distinct families of Z-line targeting modules in the COOH-terminal region of nebulin.The Journal of cell biology · 2000Article
- The NH2 terminus of titin spans the Z-disc: its interaction with a novel 19-kD ligand (T-cap) is required for sarcomeric integrity.The Journal of cell biology · 1998Article
- A primary cell culture model for defective cardiac myofibrillogenesis in Mexican axolotl embryos.In vitro cellular & developmental biology. Animal · 1997Article
- Titin expression as an early indication of heart and skeletal muscle differentiation in vitro. Developmental re-organisation in relation to cytoskeletal constituents.Journal of muscle research and cell motility · 1996Article
- Dominant negative effect of cytoplasmic actin isoproteins on cardiomyocyte cytoarchitecture and function.The Journal of cell biology · 1995Article
- Mechanisms of thin filament assembly in embryonic chick cardiac myocytes: tropomodulin requires tropomyosin for assembly.The Journal of cell biology · 1995Article
- Phosphorylation of KSP motifs in the C-terminal region of titin in differentiating myoblasts.The EMBO journal · 1993Article
- The vinculin/sarcomeric-alpha-actinin/alpha-actin nexus in cultured cardiac myocytes.The Journal of cell biology · 1992Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myofibrillogenesis was studied in cultured chick cardiomyocytes using indirect immunofluorescence microscopy and antibodies against alpha- and gamma-actin, muscle and nonmuscle tropomyosin, muscle myosin, and titin. Initially, cardiomyocytes, devoid of myofibrils, developed variable numbers of stress fiber-like structures with uniform staining for anti-muscle and nonmuscle actin and tropomyosin, and diffuse, weak staining with anti-titin. Anti-myosin labeled bundles of filaments that exhibited variable degrees of association with the stress fiber-like structures. Myofibrillogenesis occurred with a progressive, and generally simultaneous, longitudinal reorganization of stress fiber-like structures to form primitive sarcomeric units. Titin appeared to attain its mature pattern before the other major contractile proteins. Changes in the staining patterns of actin, tropomyosin, and myosin as myofibrils matured were interpreted as due to longitudinal filament alignment occurring before ordering in the axial direction. Non-muscle actin and tropomyosin were found with sarcomeric periodicity in the initial stages of sarcomere myofibrillogenesis, although their staining patterns were not identical. The localization of the "sarcomeric" proteins alpha-actin and muscle tropomyosin in stress fiber-like structures and the incorporation of non-muscle proteins in the initial stages of sarcomere organization bring into question the meaning of "sarcomeric" proteins in regard to myofibrillogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.