ArticleViral immunology2008
Quantitative recovery of scrapie agent with minimal protein from highly infectious cultures.
Article in Viral immunology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- Proteomic analysis of host brain components that bind to infectious particles in Creutzfeldt-Jakob disease.Proteomics · 2015Article
- Rapid chemical decontamination of infectious CJD and scrapie particles parallels treatments known to disrupt microbes and biofilms.Virulence · 2015Article
- Highly infectious CJD particles lack prion protein but contain many viral-linked peptides by LC-MS/MS.Journal of cellular biochemistry · 2014Article
- High CJD infectivity remains after prion protein is destroyed.Journal of cellular biochemistry · 2011Article
- Nuclease resistant circular DNAs copurify with infectivity in scrapie and CJD.Journal of neurovirology · 2011Article
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- Proliferative arrest of neural cells induces prion protein synthesis, nanotube formation, and cell-to-cell contacts.Journal of cellular biochemistry · 2010Article
- The physical relationship between infectivity and prion protein aggregates is strain-dependent.PLoS pathogens · 2010Article
- Agent-specific Shadoo responses in transmissible encephalopathies.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2010Article
- The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt-Jakob disease and scrapie agents.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Prion strain discrimination based on rapid in vivo amplification and analysis by the cell panel assay.PloS one · 2009Article
- Disease-specific motifs can be identified in circulating nucleic acids from live elk and cattle infected with transmissible spongiform encephalopathies.Nucleic acids research · 2009Article
- Strain-specific viral properties of variant Creutzfeldt-Jakob disease (vCJD) are encoded by the agent and not by host prion protein.Journal of cellular biochemistry · 2009Article
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Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
There are few reports on the isolation, quantitative recovery, and relative purification of infectious particles that cause scrapie, Creutzfeldt-Jakob disease (CJD) and epidemic bovine spongiform encephalopathy (BSE). Because pure prion protein (PrP) has failed to show significant infectivity, it is critical to find other molecules that are integral agent components. Only complex diseased tissues such as degenerating brain have been fractionated, and agent recoveries have been quite low in concentrated abnormal prion protein (PrP-res) preparations. To simplify the purification of infectious particles, we evaluated a monotypic cell line that continuously produced high levels of the 22L scrapie agent (N2a-22L). A new rapid and accurate GT1 culture assay was used to titrate infectivity in six representative sucrose gradients. We developed a streamlined approximately 3-h procedure that yielded full recovery of starting infectivity in fractions with only a few selected protein bands (representing <1% of starting protein). Infectious particles reproducibly sedimented through >30% sucrose steps, whereas PrP and PrP-res sedimentation varied depending on the conditions used. Both normal and abnormal PrP could be largely separated from infectivity in a single short centrifugation. Because no foreign enzymes were added to achieve reasonably purified infectious particles, these preparations may be used to elicit diagnostic antibodies to foreign agent proteins.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.