ArticleProceedings of the National Academy of Sciences of the United States of America2008
Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Modeling and targeting general and chromosome-specific aneuploidy in cancer.Genes & development · 2025Review
- A Study on Potential Sources of Perineuronal Net-Associated Sema3A in Cerebellar Nuclei Reveals Toxicity of Non-Invasive AAV-Mediated Cre Expression in the Central Nervous System.International journal of molecular sciences · 2025Article
- Modeling specific aneuploidies: from karyotype manipulations to biological insights.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Review
- Article
- Sexual Dimorphism through the Lens of Genome Manipulation, Forward Genetics, and Spatiotemporal Sequencing.Genome biology and evolution · 2021Article
- Site-Specific Recombination with Inverted Target Sites: A Cautionary Tale of Dicentric and Acentric Chromosomes.Genetics · 2020Article
- Versatile cell ablation tools and their applications to study loss of cell functions.Cellular and molecular life sciences : CMLS · 2019Review
- Whole chromosome loss and associated breakage-fusion-bridge cycles transform mouse tetraploid cells.The EMBO journal · 2018Article
- Creating conditional dual fluorescence labeled transgenic animals for studying function of small noncoding RNAs.Connective tissue research · 2017Article
- Adult pituitary cell maintenance: lineage-specific contribution of self-duplication.Molecular endocrinology (Baltimore, Md.) · 2013Article
- Recruitment of 5' Hoxa genes in the allantois is essential for proper extra-embryonic function in placental mammals.Development (Cambridge, England) · 2012Article
- Cre-mediated recombination can induce apoptosis in vivo by activating the p53 DNA damage-induced pathway.Genesis (New York, N.Y. : 2000) · 2012Article
- A non-specific effect associated with conditional transgene expression based on Cre-loxP strategy in mice.PloS one · 2011Article
- Generation and analysis of partially haploid cells with Cre-mediated chromosome deletion in the lymphoid system.The Journal of biological chemistry · 2010Article
- Divergent transcriptional activities determine limb identity.Nature communications · 2010Article
- A mouse model of osteochondromagenesis from clonal inactivation of Ext1 in chondrocytes.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Gene Editing in Human Pluripotent Stem Cells: Choosing the Correct Path.Journal of stem cell and regenerative biologyArticle
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The loxP/Cre recombination system is a widely used tool for mouse functional genomics, in particular for in vivo conditional mutagenesis. Depending on the relative orientation and position of loxP sites, Cre-mediated recombination can result in a variety of targeted genomic rearrangements. It was previously reported that loss of the loxP-carrying chromosome can occur when loxP sites are arranged in inverse orientation. By using a chromosome 2 carrying inverted loxP sites, we found that Cre-mediated recombination not only causes chromosomal loss but also triggers apoptosis. We show that targeted recombination between inverted loxP sites (TRIP) triggers cell death specifically in proliferating Cre-expressing cells, and we provide evidence that TRIP is an efficient tool to ablate proliferating cells within genetically defined cell populations. Furthermore, the procedure requires only a simple, one-step intercross but neither the use of toxins nor the additional step of prodrug injection. With the large repertoire of tissue-specific or inducible Cre-expressing transgenes available, TRIP-mediated cell ablation is valuable to investigate the function of a large variety of cell populations in the context of a whole organism, which includes mechanisms underlying organ development and tissue homeostasis.
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