ArticleReproduction (Cambridge, England)2008
Beta-defensin 22 is a major component of the mouse sperm glycocalyx.
Article in Reproduction (Cambridge, England), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
- Ovochymase 2 is a key regulatory factor modulating proteolytic pathways and sperm maturation in the mammalian epididymis†.Biology of reproduction · 2025Article
- Beta-defensin 126 (DEFB126) localization and expression in the sperms and male reproductive tract of the dromedary camel (Open veterinary journal · 2025Article
- β-Defensin gene copy number variation in cattle.Royal Society open science · 2024Article
- Defensins: defenders of human reproductive health.Human reproduction update · 2023Review
- Mysteries and unsolved problems of mammalian fertilization and related topics.Biology of reproduction · 2022Review
- Toll-like receptor-4 null mutation causes fetal loss and fetal growth restriction associated with impaired maternal immune tolerance in mice.Scientific reports · 2021Article
- Buffalo sperm surface proteome profiling reveals an intricate relationship between innate immunity and reproduction.BMC genomics · 2021Article
- Unusual interplay of contrasting selective pressures on β-defensin genes implicated in male fertility of the Buffalo (Bubalus bubalis).BMC evolutionary biology · 2019Article
- Eukaryotic protein glycosylation: a primer for histochemists and cell biologists.Histochemistry and cell biology · 2017Review
- Conserved gene expression in sperm reservoirs between birds and mammals in response to mating.BMC genomics · 2017Article
- Cauda Epididymis-Specific Beta-Defensin 126 Promotes Sperm Motility but Not Fertilizing Ability in Cattle.Biology of reproduction · 2016Article
- Sperm-Coating Beta-Defensin 126 Is a Dissociation-Resistant Dimer Produced by Epididymal Epithelium in the Bovine Reproductive Tract.Biology of reproduction · 2016Article
- Lectin binding of human sperm associates with DEFB126 mutation and serves as a potential biomarker for subfertility.Scientific reports · 2016Article
- Progesterone Accelerates the Completion of Sperm Capacitation and Activates CatSper Channel in Spermatozoa from the Rhesus Macaque.Biology of reproduction · 2015Article
- Sperm Proteome Maturation in the Mouse Epididymis.PloS one · 2015Article
- Multifunctional glycoprotein DEFB126--a curious story of defensin-clad spermatozoa.Nature reviews. Urology · 2012Review
- Antimicrobial peptides: agents of border protection for companion animals.Veterinary dermatology · 2012Review
- β-Defensins: multifunctional modulators of infection, inflammation and more?Journal of innate immunity · 2012Review
- Reproductive tissue-specific expression profiling and genetic variation across a 19 gene bovine β-defensin cluster.Immunogenetics · 2011Article
- A common mutation in the defensin DEFB126 causes impaired sperm function and subfertility.Science translational medicine · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Surface components of sperm isolated from the cauda epididymides were stabilized by whole sperm fixation for immunization of rabbits. The resulting immunoglobulins (Igs) recognized a single protein of 130 kDa (non-reduced) or 54-57 kDa (reduced) on western blots of cauda sperm. Igs recognized the same 54-57 kDa protein band on whole tissue blots of the corpus and cauda epididymidis and vas deferens. No immunoreactive bands were detected on blots of the prostate, seminal vesicles, testes, caput epididymis, or any of various non-reproductive tissues. Removal of sperm from the vas deferens prior to blotting eliminated the detection of the sperm antigen. Antibodies raised to synthetic peptides, identical in amino acid sequence to two unique spans of DEFB22, recognized the same 130/54-57 kDa antigen on western blots of both caudal sperm and the purified antigen isolated with the anti-sperm Ig. From indirect immunofluorescence, both the anti-sperm and anti-peptide Igs appeared to localize to the entire sperm surface, a pattern confirmed at the ultrastructural level. Real-time PCR identified the corpus epididymides as the major site of expression of DEFB22, with negligible expression in the testes, caput epididymides, and vas deferens. Immunostaining of epididymal sections showed DEFB22 being released into the lumen at the distal caput/proximal corpus, with sperm becoming intensely coated with DEFB22 as they reached the distal corpus. Most uterine sperm recovered from mice 4 h following copulation exhibited DEFB22 coating the entire sperm surface. By contrast, some sperm recovered from the oviduct and cumulus extracellular matrix showed loss of DEFB22 from the sperm head.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.