ArticleJournal of molecular biology2008
Localization of the N-terminus of minor coat protein IIIa in the adenovirus capsid.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Adenoviral Vector System: A Comprehensive Overview of Constructions, Therapeutic Applications and Host Responses.Journal of microbiology (Seoul, Korea) · 2024Review
- RANBP2 and USP9x regulate nuclear import of adenovirus minor coat protein IIIa.PLoS pathogens · 2022Article
- Viral vector platforms within the gene therapy landscape.Signal transduction and targeted therapy · 2021Review
- Structural Organization and Protein-Protein Interactions in Human Adenovirus Capsid.Sub-cellular biochemistry · 2021Review
- Progress in Adenoviral Capsid-Display Vaccines.Biomedicines · 2018Review
- Cryo-EM structure of human adenovirus D26 reveals the conservation of structural organization among human adenoviruses.Science advances · 2017Article
- Astrocytes promote progression of breast cancer metastases to the brain via a KISS1-mediated autophagy.Autophagy · 2017Article
- Adenoviral vector-based strategies against infectious disease and cancer.Human vaccines & immunotherapeutics · 2016Review
- Structures of Adenovirus Incomplete Particles Clarify Capsid Architecture and Show Maturation Changes of Packaging Protein L1 52/55k.Journal of virology · 2015Article
- Review
- Structures and organization of adenovirus cement proteins provide insights into the role of capsid maturation in virus entry and infection.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Regulation of a viral proteinase by a peptide and DNA in one-dimensional space: I. binding to DNA AND to hexon of the precursor to protein VI, pVI, of human adenovirus.The Journal of biological chemistry · 2013Article
- Latest insights on adenovirus structure and assembly.Viruses · 2012Review
- Chapter two--Adenovirus strategies for tissue-specific targeting.Advances in cancer research · 2012Review
- Adenovirus structural protein IIIa is involved in the serotype specificity of viral DNA packaging.Journal of virology · 2011Article
- Unnatural amino acid incorporation onto adenoviral (Ad) coat proteins facilitates chemoselective modification and retargeting of Ad type 5 vectors.Journal of virology · 2011Article
- Characterization of Empty adenovirus particles assembled in the absence of a functional adenovirus IVa2 protein.Journal of virology · 2011Article
- A genetic strategy for combined screening and localized imaging of breast cancer.Molecular imaging and biology · 2011Article
- Noninvasive monitoring of mRFP1- and mCherry-labeled oncolytic adenoviruses in an orthotopic breast cancer model by spectral imaging.Molecular imaging · 2010Article
- Adenovirus.Current topics in microbiology and immunology · 2010Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Minor coat protein IIIa is conserved in all adenoviruses (Ads) and is required for correct viral assembly, but its precise function in capsid organization is unknown. The latest Ad capsid model proposes that IIIa is located underneath the vertex region. To obtain experimental evidence on the location of IIIa and to further define its role, we engineered the IIIa gene to encode heterologous N-terminal peptide extensions. Recombinant Ad variants with IIIa encoding six-histidine (6His) tag, 6His, and FLAG peptides, or with 6His linked to FLAG with a (Gly(4)Ser)(3) linker were rescued and analyzed for virus yield, capsid incorporation of heterologous peptides, and capsid stability. Longer extensions could not be rescued. Western blot analysis confirmed that the modified IIIa proteins were expressed in infected cells and incorporated into virions. In the Ad encoding the 6His-linker-FLAG-IIIa gene, the 6His tag was present in light particles, but not in mature virions. Immunoelectron microscopy of this virus showed that the FLAG epitope is not accessible to antibodies on the viral particles. Three-dimensional electron microscopy and difference mapping located the IIIa N-terminal extension beneath the vertex complex, wedged at the interface between the penton base and peripentonal hexons, therefore supporting the latest proposed model. The position of the IIIa N-terminus and its low tolerance for modification provide new clues for understanding the role of this minor coat protein in Ad capsid assembly and disassembly.
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