ArticleBMC genomics2008
Identification of direct regulatory targets of the transcription factor Sox10 based on function and conservation.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Gtf2i-encoded transcription factor Tfii-i regulates myelination via Sox10 and Mbp regulatory elements.Nature communications · 2025Article
- Deletion ofBiomedicines · 2023Article
- A Novel Role for Oligodendrocyte Precursor Cells (OPCs) and Sox10 in Mediating Cellular and Behavioral Responses to Heroin.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018Article
- SOX10 is over-expressed in bladder cancer and contributes to the malignant bladder cancer cell behaviors.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2017Article
- Stringent comparative sequence analysis reveals SOX10 as a putative inhibitor of glial cell differentiation.BMC genomics · 2016Article
- Sox10 expression in ovarian epithelial tumors is associated with poor overall survival.Virchows Archiv : an international journal of pathology · 2016Article
- Regulation of SOX10 stability via ubiquitination-mediated degradation by Fbxw7α modulates melanoma cell migration.Oncotarget · 2015Article
- Nox4-mediated cell signaling regulates differentiation and survival of neural crest stem cells.Molecules and cells · 2014Article
- Haplotype-specific modulation of a SOX10/CREB response element at the Charcot-Marie-Tooth disease type 4C locus SH3TC2.Human molecular genetics · 2014Article
- RNAi silencing of the SoxE gene suppresses cell proliferation in silkworm BmN4 cells.Molecular biology reports · 2014Article
- Down-regulation of Sox11 is required for efficient osteogenic differentiation of adipose-derived stem cells.Molecules and cells · 2014Article
- Modeling the neurovascular niche: unbiased transcriptome analysis of the murine subventricular zone in response to hypoxic insult.PloS one · 2013Article
- Genome-wide analysis of EGR2/SOX10 binding in myelinating peripheral nerve.Nucleic acids research · 2012Article
- SOX10 regulates expression of the SH3-domain kinase binding protein 1 (Sh3kbp1) locus in Schwann cells via an alternative promoter.Molecular and cellular neurosciences · 2012Article
- Sox10 controls migration of B16F10 melanoma cells through multiple regulatory target genes.PloS one · 2012Article
- Uncovering the transcriptional circuitry in skeletal muscle regeneration.Mammalian genome : official journal of the International Mammalian Genome Society · 2011Article
- Sox proteins in melanocyte development and melanoma.Pigment cell & melanoma research · 2010Review
- Comparative analysis of MTF-1 binding sites between human and mouse.Mammalian genome : official journal of the International Mammalian Genome Society · 2010Article
- Comparison of melanoblast expression patterns identifies distinct classes of genes.Pigment cell & melanoma research · 2009Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSox10, a member of the Sry-related HMG-Box gene family, is a critical transcription factor for several important cell lineages, most notably the neural crest stem cells and the derivative peripheral glial cells and melanocytes. Thus far, only a handful of direct target genes are known for this transcription factor limiting our understanding of the biological network it governs.
resultsWe describe identification of multiple direct regulatory target genes of Sox10 through a procedure based on function and conservation. By combining RNA interference technique and DNA microarray technology, we have identified a set of genes that show significant down-regulation upon introduction of Sox10 specific siRNA into Schwannoma cells. Subsequent comparative genomics analyses led to potential binding sites for Sox10 protein conserved across several mammalian species within the genomic region proximal to these genes. Multiple sites belonging to 4 different genes (proteolipid protein, Sox10, extracellular superoxide dismutase, and pleiotrophin) were shown to directly interact with Sox10 by chromatin immunoprecipitation assay. We further confirmed the direct regulation through the identified cis-element for one of the genes, extracellular superoxide dismutase, using electrophoretic mobility shift assay and reporter assay.
conclusionIn sum, the process of combining differential expression profiling and comparative genomics successfully led to further defining the role of Sox10, a critical transcription factor for the development of peripheral glia. Our strategy utilizing relatively accessible techniques and tools should be applicable to studying the function of other transcription factors.
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