ArticleBMC molecular biology2008
Evaluation of suitable reference genes for gene expression studies in bovine muscular tissue.
Article in BMC molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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52 citing papers in PubMed.
- Myokines Produced by Cultured Bovine Satellite Cells Harvested from 3- and 11-Month-Old Angus Steers.Animals : an open access journal from MDPI · 2024Article
- Two novel SNPs identified inAnimal biotechnology · 2023Article
- Heat shock protein 27 regulates myogenic and self-renewal potential of bovine satellite cells under heat stress.Journal of animal science · 2023Article
- Selection and validation of reference genes for the normalization of quantitative real-time PCR in different muscle tissues of rabbits.BMC zoology · 2022Article
- Reference gene screening for analyzing gene expression in the heart, liver, spleen, lung and kidney of forest musk deer.The Journal of veterinary medical science · 2021Article
- Validation of reference genes for use in untreated bovine fibroblasts.Scientific reports · 2021Article
- Implication of the NLRP3 Inflammasome in Bovine Age-Related Sarcopenia.International journal of molecular sciences · 2021Article
- Validation of Reference Genes via qRT-PCR in Multiple Conditions in Brandt's Voles,Animals : an open access journal from MDPI · 2021Article
- Optimizations for identifying reference genes in bone and cartilage bioengineering.BMC biotechnology · 2021Article
- The Impact of Polyamine Precursors, Polyamines, and Steroid Hormones on Temporal Messenger RNA Abundance in Bovine Satellite Cells Induced to Differentiate.Animals : an open access journal from MDPI · 2021Article
- TATA box binding protein and ribosomal protein 4 are suitable reference genes for normalization during quantitative polymerase chain reaction study in bovine mesenchymal stem cells.Asian-Australasian journal of animal sciences · 2020Article
- Transcriptomic profile of semitendinosus muscle of bulls of different breed and performance.Journal of applied genetics · 2020Article
- Transcriptomic Profile of Primary Culture of Skeletal Muscle Cells Isolated from Semitendinosus Muscle of Beef and Dairy Bulls.International journal of molecular sciences · 2020Article
- Stable reference genes for expression studies in breast muscle of normal and white striping-affected chickens.Molecular biology reports · 2020Article
- Reference gene selection and myosin heavy chain (MyHC) isoform expression in muscle tissues of domestic yak (Bos grunniens).PloS one · 2020Article
- The Selection of Reference Genes for Quantitative Real-Time PCR in the Ashidan Yak Mammary Gland During Lactation and Dry Period.Animals : an open access journal from MDPI · 2019Article
- Article
- Identification of endogenous normalizing genes for expression studies in inguinal ring tissue for scrotal hernias in pigs.PloS one · 2018Article
- The effect of Bu Zhong Yi Qi decoction on simulated weightlessness‑induced muscle atrophy and its mechanisms.Molecular medicine reports · 2017Article
- The CaNature communications · 2017Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundReal-time reverse transcriptase quantitative polymerase chain reaction (real-time RTqPCR) is a technique used to measure mRNA species copy number as a way to determine key genes involved in different biological processes. However, the expression level of these key genes may vary among tissues or cells not only as a consequence of differential expression but also due to different factors, including choice of reference genes to normalize the expression levels of the target genes; thus the selection of reference genes is critical for expression studies. For this purpose, ten candidate reference genes were investigated in bovine muscular tissue.
resultsThe value of stability of ten candidate reference genes included in three groups was estimated: the so called 'classical housekeeping' genes (18S, GAPDH and ACTB), a second set of genes used in expression studies conducted on other tissues (B2M, RPII, UBC and HMBS) and a third set of novel genes (SF3A1, EEF1A2 and CASC3). Three different statistical algorithms were used to rank the genes by their stability measures as produced by geNorm, NormFinder and Bestkeeper. The three methods tend to agree on the most stably expressed genes and the least in muscular tissue. EEF1A2 and HMBS followed by SF3A1, ACTB, and CASC3 can be considered as stable reference genes, and B2M, RPII, UBC and GAPDH would not be appropriate. Although the rRNA-18S stability measure seems to be within the range of acceptance, its use is not recommended because its synthesis regulation is not representative of mRNA levels.
conclusionBased on geNorm algorithm, we propose the use of three genes SF3A1, EEF1A2 and HMBS as references for normalization of real-time RTqPCR in muscle expression studies.
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