ArticleMolecular microbiology2008
Archaeal RNA polymerase subunits E and F are not required for transcription in vitro, but a Thermococcus kodakarensis mutant lacking subunit F is temperature-sensitive.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 45 citations in OpenAlex.
- The Thermococcales as a model system: historical perspectives and emerging tools.Journal of bacteriology · 2025Review
- Archaea as a Model System for Molecular Biology and Biotechnology.Biomolecules · 2023Review
- Direct binding of TFEα opens DNA binding cleft of RNA polymerase.Nature communications · 2020Article
- Archaeal transcription.Transcription · 2020Review
- FttA is a CPSF73 homologue that terminates transcription in Archaea.Nature microbiology · 2020Article
- An overview of 25 years of research on Thermococcus kodakarensis, a genetically versatile model organism for archaeal research.Folia microbiologica · 2020Review
- Identification of a radical SAM enzyme involved in the synthesis of archaeosine.Nature chemical biology · 2019Article
- Distinct Modified Nucleosides in tRNAJournal of bacteriology · 2019Article
- The Role of Archaeal Chromatin in Transcription.Journal of molecular biology · 2019Review
- Article
- Distinct Physiological Roles of the Three Ferredoxins Encoded in the Hyperthermophilic ArchaeonmBio · 2019Article
- The Cdc45/RecJ-like protein forms a complex with GINS and MCM, and is important for DNA replication in Thermococcus kodakarensis.Nucleic acids research · 2017Article
- Factor-dependent archaeal transcription termination.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Engineering of the Hyperthermophilic Archaeon Thermococcus kodakarensis for Chitin-Dependent Hydrogen Production.Applied and environmental microbiology · 2017Article
- Genetic analyses of the functions of [NiFe]-hydrogenase maturation endopeptidases in the hyperthermophilic archaeon Thermococcus kodakarensis.Extremophiles : life under extreme conditions · 2017Article
- Transcription Regulation in Archaea.Journal of bacteriology · 2016Review
- Molecular Mechanisms of Transcription Initiation-Structure, Function, and Evolution of TFE/TFIIE-Like Factors and Open Complex Formation.Journal of molecular biology · 2016Review
- Analyses of in vivo interactions between transcription factors and the archaeal RNA polymerase.Methods (San Diego, Calif.) · 2015Article
- Overproduction of the membrane-bound [NiFe]-hydrogenase in Thermococcus kodakarensis and its effect on hydrogen production.Frontiers in microbiology · 2015Article
- The X-ray crystal structure of the euryarchaeal RNA polymerase in an open-clamp configuration.Nature communications · 2014Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
All archaeal genomes encode RNA polymerase (RNAP) subunits E and F that share a common ancestry with the eukaryotic RNAP subunits A43 and A14 (Pol I), Rpb7 and Rpb4 (Pol II), and C25 and C17 (Pol III). By gene replacement, we have isolated archaeal mutants of Thermococcus kodakarensis with the subunit F-encoding gene (rpoF) deleted, but we were unable to isolate mutants lacking the subunit E-encoding gene (rpoE). Wild-type T. kodakarensis grows at temperatures ranging from 60 degrees C to 100 degrees C, optimally at 85 degrees C, and the DeltarpoF cells grew at the same rate as wild type at 70 degrees C, but much slower and to lower cell densities at 85 degrees C. The abundance of a chaperonin subunit, CpkB, was much reduced in the DeltarpoF strain growing at 85 degrees C and increased expression of cpkB, rpoF or rpoE integrated at a remote site in the genome, using a nutritionally regulated promoter, improved the growth of DeltarpoF cells. RNAP preparations purified from DeltarpoF cells lacked subunit F and also subunit E and a transcription factor TFE that co-purifies with RNAP from wild-type cells, but in vitro, this mutant RNAP exhibited no discernible differences from wild-type RNAP in promoter-dependent transcription, abortive transcript synthesis, transcript elongation or termination.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.