ArticleThe Plant journal : for cell and molecular biology2009
An antioxidant redox system in the nucleus of wheat seed cells suffering oxidative stress.
Article in The Plant journal : for cell and molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 122 citations in OpenAlex.
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- Transient drought during flowering modifies the grain proteome of bread winter wheat.Frontiers in plant science · 2023Article
- Reactive oxygen species signalling in plant stress responses.Nature reviews. Molecular cell biology · 2022Review
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- Comparative Proteomics at the Critical Node of Vigor Loss in Wheat Seeds Differing in Storability.Frontiers in plant science · 2021Article
- The Rapid Cytological Process of Grain Determines Early Maturity in Weedy Rice.Frontiers in plant science · 2021Article
- What Do We Know About the Genetic Basis of Seed Desiccation Tolerance and Longevity?International journal of molecular sciences · 2020Review
- The Significance of Reactive Oxygen Species and Antioxidant Defense System in Plants: A Concise Overview.Frontiers in plant science · 2020Review
- Thioredoxin Network in Plant Mitochondria: Cysteine S-Posttranslational Modifications and Stress Conditions.Frontiers in plant science · 2020Review
- Non-Mammalian Prdx6 Enzymes (Proteins with 1-Cys Prdx Mechanism) Display PLA₂ Activity Similar to the Human Orthologue.Antioxidants (Basel, Switzerland) · 2019Article
- Seeds and the Art of Genome Maintenance.Frontiers in plant science · 2019Review
- On the role of the plant mitochondrial thioredoxin system during abiotic stress.Plant signaling & behavior · 2019Article
- Involvement of Glutaredoxin and Thioredoxin Systems in the Nitrogen-Fixing Symbiosis between Legumes and Rhizobia.Antioxidants (Basel, Switzerland) · 2018Review
- Peroxiredoxins and Redox Signaling in Plants.Antioxidants & redox signaling · 2018Review
- Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.Redox biology · 2017Article
- Mitochondrial AtTrxo1 is transcriptionally regulated by AtbZIP9 and AtAZF2 and affects seed germination under saline conditions.Journal of experimental botany · 2017Article
- The Plant Immunity Regulating F-Box ProteinFrontiers in plant science · 2017Article
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cereal seed cells contain different mechanisms for protection against the oxidative stress that occurs during maturation and germination. One such mechanism is based on the antioxidant activity of a 1-Cys peroxiredoxin (1-Cys Prx) localized in the nuclei of aleurone and scutellum cells. However, nothing is known about the mechanism of activation of this enzyme. Here, we describe the pattern of localization of NADPH thioredoxin reductase (NTR) in developing and germinating wheat seeds using an immunocytochemical analysis. The presence of NTR in transfer cells, vascular tissue, developing embryo and root meristematic cells, agrees with the localization of thioredoxin h (Trx h), and supports the important function of the NTR/Trx system in cell proliferation and communication. Interestingly, NTR is found in the nuclei of seed cells suffering oxidative stress, thus showing co-localization with Trx h and 1-Cys Prx. To test whether the NTR/Trx system serves as a reductant of the 1-Cys Prx, we cloned a full-length cDNA encoding 1-Cys Prx from wheat, and expressed the recombinant protein in Escherichia coli. Using the purified components, we show NTR-dependent activity of the 1-Cys Prx. Mutants of the 1-Cys Prx allowed us to demonstrate that the peroxidatic residue of the wheat enzyme is Cys46, which is overoxidized in vitro under oxidant conditions. Analysis of extracts from developing and germinating seeds confirmed 1-Cys Prx overoxidation in vivo. Based on these results, we propose that NADPH is the source of the reducing power to regenerate 1-Cys Prx in the nuclei of seed cells suffering oxidative stress, in a process that is catalyzed by NTR.
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