ArticleTraffic (Copenhagen, Denmark)2008
Acute perturbations in Golgi organization impact de novo sphingomyelin synthesis.
Article in Traffic (Copenhagen, Denmark), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- The Infectious Bronchitis Coronavirus Envelope Protein Alters Golgi pH To Protect the Spike Protein and Promote the Release of Infectious Virus.Journal of virology · 2019Article
- Commonly used trafficking blocks disrupt ARF1 activation and the localization and function of specific Golgi proteins.Molecular biology of the cell · 2018Article
- Brefeldin A promotes the appearance of oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells.Journal of lipid research · 2016Article
- 2-Deoxy-D-glucose treatment changes the Golgi apparatus architecture without blocking synthesis of complex lipids.Histochemistry and cell biology · 2015Article
- PKD controls mitotic Golgi complex fragmentation through a Raf-MEK1 pathway.Molecular biology of the cell · 2013Article
- Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells.Molecular & cellular proteomics : MCP · 2012Article
- Inactivation of ceramide transfer protein during pro-apoptotic stress by Golgi disassembly and caspase cleavage.The Biochemical journal · 2012Article
- Chlamydia trachomatis co-opts GBF1 and CERT to acquire host sphingomyelin for distinct roles during intracellular development.PLoS pathogens · 2011Article
- Golgi glycosylation and human inherited diseases.Cold Spring Harbor perspectives in biology · 2011Review
- The lipid transfer protein CERT interacts with the Chlamydia inclusion protein IncD and participates to ER-Chlamydia inclusion membrane contact sites.PLoS pathogens · 2011Article
- The ceramide-enriched trans-Golgi compartments reorganize together with other parts of the Golgi apparatus in response to ATP-depletion.Histochemistry and cell biology · 2011Article
- Cog2 null mutant CHO cells show defective sphingomyelin synthesis.The Journal of biological chemistry · 2010Article
- Rab6 and Rab11 regulate Chlamydia trachomatis development and golgin-84-dependent Golgi fragmentation.PLoS pathogens · 2009Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The mammalian Golgi apparatus is composed of multiple stacks of cisternal membranes organized laterally into a ribbon-like structure, with close apposition of trans Golgi regions with specialized endoplasmic reticulum (ER) membranes. These contacts may be the site of ceramide transfer from its site of synthesis (ER) to sphingomyelin (SM) synthase through ceramide transfer protein (CERT). CERT extracts ceramide from the ER and transfers it to Golgi membranes but the role of overall Golgi structure in this process is unknown. We show here that localization of CERT in puncta around the Golgi complex requires both ER- and Golgi-binding domains of CERT. To examine how Golgi structure contributes to SM synthesis, we treated cells with Golgi-perturbing drugs and measured newly synthesized SM. Interestingly, disruption of Golgi morphology with nocodazole, but not ilimaquinone inhibited SM synthesis. Decreased localization of CERT with a Golgi marker correlated with decreased SM synthesis. We propose that some Golgi structural perturbations interfere with efficient ceramide trafficking through CERT, and thus SM synthesis. The organization of the mammalian Golgi ribbon together with CERT may promote specific ER-Golgi interactions for efficient delivery of ceramide for SM synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.