ArticleJournal of the Association for Research in Otolaryngology : JARO2008
Sparc protein is required for normal growth of zebrafish otoliths.
Article in Journal of the Association for Research in Otolaryngology : JARO, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
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- Lattice Shrinkage by Incorporation of Recombinant Starmaker-Like Protein within Bioinspired Calcium Carbonate Crystals.Chemistry (Weinheim an der Bergstrasse, Germany) · 2019Article
- Vestibular dysfunction, altered macular structure and trait localization in A/J inbred mice.Mammalian genome : official journal of the International Mammalian Genome Society · 2015Article
- Otolith tethering in the zebrafish otic vesicle requires Otogelin and α-Tectorin.Development (Cambridge, England) · 2015Article
- Mechanisms of otoconia and otolith development.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Review
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- Association of serum SPARC level with severity of coronary artery lesion in type 2 diabetic patients with coronary heart disease.International journal of clinical and experimental medicine · 2015Article
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- Knockdown of SPARC leads to decreased cell-cell adhesion and lens cataracts during post-gastrula development in Xenopus laevis.Development genes and evolution · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Otoliths and the homologous otoconia in the inner ear are essential for balance. Their morphogenesis is less understood than that of other biominerals, such as bone, and only a small number of their constituent proteins have been characterized. As a novel approach to identify unknown otolith proteins, we employed shotgun proteomics to analyze crude extracts from trout and catfish otoliths. We found three proteins that had not been associated previously with otolith or otoconia formation: 'Secreted acidic cysteine rich glycoprotein' (Sparc), an important bone protein that binds collagen and Ca(2+); precerebellin-like protein, which contains a C1q domain and may associate with the collagenous otolin-1 during its assembly into a framework; and neuroserpin, a serine protease inhibitor that may regulate local protease activity during framework assembly. We then used the zebrafish to investigate whether Sparc plays a role in otolith morphogenesis. Immunodetection demonstrated that Sparc is a true constituent of otoliths. Knockdown of Sparc expression in morphant zebrafish resulted in four principal types of defective otoliths: smaller, extra and ectopic, missing and fused, or completely absent. Smaller size was the predominant phenotype and independent of the severity of otic-vesicle defects. These results suggested that Sparc is directly required for normal otolith growth.
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Registered trials
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