ArticlePlant physiology2008
Different hormonal regulation of cellular differentiation and function in nucellar projection and endosperm transfer cells: a microdissection-based transcriptome study of young barley grains.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 111 citations in OpenAlex.
- Physiological and Molecular Mechanisms of Nitrogen Regulation on Grain Quality in Cereal Crops at Later Stages.International journal of molecular sciences · 2026Review
- Transport and spatio-temporal conversion of sugar facilitate the formation of spatial gradients of starch in wheat caryopses.Communications biology · 2024Article
- SWEET11b transports both sugar and cytokinin in developing barley grains.The Plant cell · 2023Article
- Dissection of Developmental Programs and Regulatory Modules Directing Endosperm Transfer Cell and Aleurone Identity in the Syncytial Endosperm of Barley.Plants (Basel, Switzerland) · 2023Article
- Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells inCells · 2022Article
- Laser microdissection transcriptome data derived gene regulatory networks of developing rice endosperm revealed tissue- and stage-specific regulators modulating starch metabolism.Plant molecular biology · 2022Article
- Programmed Cell Death in DevelopingInternational journal of molecular sciences · 2021Article
- Impact and mechanism of sulphur-deficiency on modern wheat farming nitrogen-related sustainability and gliadin content.Communications biology · 2021Article
- Grain filling in barley relies on developmentally controlled programmed cell death.Communications biology · 2021Article
- Natural Variation in Ovule Morphology Is Influenced by Multiple Tissues and Impacts Downstream Grain Development in Barley (Frontiers in plant science · 2019Article
- Carbohydrate reserves and seed development: an overview.Plant reproduction · 2018Review
- Seed tissue and nutrient partitioning, a case for the nucellus.Plant reproduction · 2018Review
- Altered Expression of the Malate-Permeable Anion Channel OsALMT4 Reduces the Growth of Rice Under Low Radiance.Frontiers in plant science · 2018Article
- Transcript Profiling Identifies NAC-Domain Genes Involved in Regulating Wall Ingrowth Deposition in Phloem Parenchyma Transfer Cells ofFrontiers in plant science · 2018Article
- A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains.Rice (New York, N.Y.) · 2017Article
- Down-regulation of the sucrose transporters HvSUT1 and HvSUT2 affects sucrose homeostasis along its delivery path in barley grains.Journal of experimental botany · 2017Article
- Silencing of ABCC13 transporter in wheat reveals its involvement in grain development, phytic acid accumulation and lateral root formation.Journal of experimental botany · 2016Article
- Increasing abscisic acid levels by immunomodulation in barley grains induces precocious maturation without changing grain composition.Journal of experimental botany · 2016Article
- Identification of Candidate Transcriptional Regulators of Epidermal Transfer Cell Development in Vicia faba Cotyledons.Frontiers in plant science · 2016Article
- Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway.Plants (Basel, Switzerland) · 2015Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucellar projection (NP) and endosperm transfer cells (ETC) are essential tissues in growing barley (Hordeum vulgare) grains, responsible for nutrient transfer from maternal to filial tissues, endosperm/embryo nutrition, and grain development. A laser microdissection pressure catapulting-based transcriptome analysis was established to study NP and ETC separately using a barley 12K macroarray. A major challenge was to isolate high-quality mRNA from preembedded, fixed tissue while maintaining tissue integrity. We show that probes generated from fixed and embedded tissue sections represent largely the transcriptome (>70%) of nonchemically treated and nonamplified references. In NP, the top-down gradient of cellular differentiation is reflected by the expression of C3HC4-type ubiquitin ligases and different histone genes, cell wall biosynthesis and expansin/extensin genes, as well as genes involved in programmed cell death-related proteolysis coupled to nitrogen remobilization, indicating distinct areas simultaneously undergoing mitosis, cell elongation, and disintegration. Activated gene expression related to gibberellin synthesis and function suggests a regulatory role for gibberellins in establishment of the differentiation gradient. Up-regulation of plasmalemma-intrinsic protein and tonoplast-intrinsic protein genes indicates involvement in nutrient transfer and/or unloading. In ETC, AP2/EREBP-like transcription factors and ethylene functions are transcriptionally activated, a response possibly coupled to activated defense mechanisms. Transcriptional activation of nucleotide sugar metabolism may be attributed to ascorbate synthesis and/or cell wall biosynthesis. These processes are potentially controlled by trehalose-6-P synthase/phosphatase, as suggested by expression of their respective genes. Up-regulation of amino acid permeases in ETC indicates important roles in active nutrient uptake from the apoplastic space into the endosperm.
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