ArticleJournal of molecular biology2008
Crystal structure of metastasis-associated protein S100A4 in the active calcium-bound form.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- The multi-faceted immune modulatory role of S100A4 in cancer and chronic inflammatory disease.Frontiers in immunology · 2025Review
- What Makes a Good Protein-Protein Interaction Stabilizer: Analysis and Application of the Dual-Binding Mechanism.ACS central science · 2023Article
- Solid-State NMR Study to Probe the Effects of Divalent Metal Ions (CaLangmuir : the ACS journal of surfaces and colloids · 2021Article
- Absence of S100A4 in the mouse lens induces an aberrant retina-specific differentiation program and cataract.Scientific reports · 2021Article
- Regulation of von-Willebrand Factor Secretion from Endothelial Cells by the Annexin A2-S100A10 Complex.International journal of molecular sciences · 2018Review
- In situ NMR measurement of macromolecule-bound metal ion concentrations.Journal of biomolecular NMR · 2016Article
- Increased S100A4 expression in the vasculature of human COPD lungs and murine model of smoke-induced emphysema.Respiratory research · 2015Article
- The solution structure of human calcium-bound S100A4 mutated at four cysteine loci.Journal of biomolecular NMR · 2015Article
- Efficient inhibition of tumor angiogenesis and growth by a synthetic peptide blocking S100A4-methionine aminopeptidase 2 interaction.Molecular therapy. Methods & clinical development · 2015Article
- The C-terminal random coil region tunes the Ca²⁺-binding affinity of S100A4 through conformational activation.PloS one · 2014Article
- Anti-S100A4 antibody suppresses metastasis formation by blocking stroma cell invasion.Neoplasia (New York, N.Y.) · 2012Article
- Cysteine 81 is critical for the interaction of S100A4 and myosin-IIA.Biochemistry · 2011Article
- Two functional S100A4 monomers are necessary for regulating nonmuscle myosin-IIA and HCT116 cell invasion.Biochemistry · 2011Article
- Design, synthesis, and structure-activity relationship exploration of 1-substituted 4-aroyl-3-hydroxy-5-phenyl-1H-pyrrol-2(5H)-one analogues as inhibitors of the annexin A2-S100A10 protein interaction.Journal of medicinal chemistry · 2011Article
- Mechanism of the Ca²+-dependent interaction between S100A4 and tail fragments of nonmuscle myosin heavy chain IIA.Journal of molecular biology · 2011Article
- Solution structure and dynamics of S100A5 in the apo and Ca2+-bound states.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2009Article
- Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
S100A4 (metastasin) is a member of the S100 family of calcium-binding proteins that is directly involved in tumorigenesis. Until recently, the only structural information available was the solution NMR structure of the inactive calcium-free form of the protein. Here we report the crystal structure of human S100A4 in the active calcium-bound state at 2.03 A resolution that was solved by molecular replacement in the space group P6(5) with two molecules in the asymmetric unit from perfectly merohedrally twinned crystals. The Ca(2+)-bound S100A4 structure reveals a large conformational change in the three-dimensional structure of the dimeric S100A4 protein upon calcium binding. This calcium-dependent conformational change opens up a hydrophobic binding pocket that is capable of binding to target proteins such as annexin A2, the tumor-suppressor protein p53 and myosin IIA. The structure of the active form of S100A4 provides insight into its interactions with its binding partners and a better understanding of its role in metastasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.