ArticleThe Journal of biological chemistry2008
Targeting of hFis1 to peroxisomes is mediated by Pex19p.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 74 citations in OpenAlex.
- A dual role of the conserved PEX19 helix in safeguarding peroxisomal membrane proteins.iScience · 2024Article
- The SUMO protease SENP3 regulates mitochondrial autophagy mediated by Fis1.EMBO reports · 2022Article
- Neuronal Protein Farnesylation Regulates Hippocampal Synaptic Plasticity and Cognitive Function.Molecular neurobiology · 2021Article
- Mitochondrial Fission Protein 1: Emerging Roles in Organellar Form and Function in Health and Disease.Frontiers in endocrinology · 2021Review
- Morphology Remodeling and Selective Autophagy of Intracellular Organelles during Viral Infections.International journal of molecular sciences · 2020Review
- Peroxisomal fission is modulated by the mitochondrial Rho-GTPases, Miro1 and Miro2.EMBO reports · 2020Article
- Organelle interplay-peroxisome interactions in health and disease.Journal of inherited metabolic disease · 2020Review
- The Ways of Tails: the GET Pathway and more.The protein journal · 2019Review
- Human Fis1 regulates mitochondrial dynamics through inhibition of the fusion machinery.The EMBO journal · 2019Article
- The Structure and Function of Acylglycerophosphate Acyltransferase 4/ Lysophosphatidic Acid Acyltransferase Delta (AGPAT4/LPAATδ).Frontiers in cell and developmental biology · 2019Review
- A bacteria-derived tail anchor localizes to peroxisomes in yeast and mammalian cells.Scientific reports · 2018Article
- Hansenula polymorpha Aat2p is targeted to peroxisomes via a novel Pex20p-dependent pathway.FEBS letters · 2018Article
- A role for Mitochondrial Rho GTPase 1 (MIRO1) in motility and membrane dynamics of peroxisomes.Traffic (Copenhagen, Denmark) · 2018Article
- Functional regions of the peroxin Pex19 necessary for peroxisome biogenesis.The Journal of biological chemistry · 2017Article
- Predicting the targeting of tail-anchored proteins to subcellular compartments in mammalian cells.Journal of cell science · 2017Article
- Peroxisomes are platforms for cytomegalovirus' evasion from the cellular immune response.Scientific reports · 2016Article
- De novo peroxisome biogenesis: Evolving concepts and conundrums.Biochimica et biophysica acta · 2016Review
- Towards the molecular mechanism of the integration of peroxisomal membrane proteins.Biochimica et biophysica acta · 2016Review
- Multiple pathways for protein transport to peroxisomes.Journal of molecular biology · 2015Review
- A new pathway for mitochondrial quality control: mitochondrial-derived vesicles.The EMBO journal · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The processes of peroxisome formation and proliferation are still a matter of debate. We have previously shown that peroxisomes share some components of their division machinery with mitochondria. hFis1, a tail-anchored membrane protein, regulates the membrane fission of both organelles by DLP1/Drp1 recruitment, but nothing is known about the mechanisms of the dual targeting of hFis1. Here we demonstrate for the first time that peroxisomal targeting of hFis1 depends on Pex19p, a peroxisomal membrane protein import factor. hFis1/Pex19p binding was demonstrated by expression and co-immunoprecipitation studies. Using mutated versions of hFis1 an essential binding region for Pex19p was located within the last 26 C-terminal amino acids of hFis1, which are required for proper targeting to both mitochondria and peroxisomes. The basic amino acids in the very C terminus are not essential for Pex19p binding and peroxisomal targeting, but are instead required for mitochondrial targeting. Silencing of Pex19p by small interference RNA reduced the targeting of hFis1 to peroxisomes, but not to mitochondria. In contrast, overexpression of Pex19p alone was not sufficient to shift the targeting of hFis1 to peroxisomes. Our findings indicate that targeting of hFis1 to peroxisomes and mitochondria are independent events and support a direct, Pex19p-dependent targeting of peroxisomal tail-anchored proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.