ArticleThe Journal of biological chemistry2008
The pathway via D-galacturonate/L-galactonate is significant for ascorbate biosynthesis in Euglena gracilis: identification and functional characterization of aldonolactonase.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 68 citations in OpenAlex.
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- Origin and evolution of signaling pathways responsible for ascorbic acid synthesis and catabolism during plant terrestrialization.Horticulture research · 2025Article
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- Transport mechanism of DgoT, a bacterial homolog of SLC17 organic anion transporters.The EMBO journal · 2024Article
- Biochemical and Functional Profiling of Thioredoxin-Dependent Cytosolic GPX-like Proteins inBiomolecules · 2024Article
- A Synthetic Biology Perspective on the Bioengineering Tools for an Industrial Microalga:Frontiers in bioengineering and biotechnology · 2022Review
- Identification of the Aldo-Keto Reductase Responsible for d-Galacturonic Acid Conversion to l-Galactonate inJournal of fungi (Basel, Switzerland) · 2021Article
- Diverse Biosynthetic Pathways and Protective Functions against Environmental Stress of Antioxidants in Microalgae.Plants (Basel, Switzerland) · 2021Review
- Genomics Reveals the Metabolic Potential and Functions in the Redistribution of Dissolved Organic Matter in Marine Environments of the GenusMicroorganisms · 2020Article
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- Metabolism of Stone Fruits: Reciprocal Contribution Between Primary Metabolism and Cell Wall.Frontiers in plant science · 2020Review
- Ascorbic acid metabolism and functions: A comparison of plants and mammals.Free radical biology & medicine · 2018Review
- Metagenomic sequencing suggests a diversity of RNA interference-like responses to viruses across multicellular eukaryotes.PLoS genetics · 2018Article
- FromFrontiers in plant science · 2018Review
- Metabolite-Centric Reporter Pathway and Tripartite Network Analysis ofFrontiers in bioengineering and biotechnology · 2018Article
- Integrated bioinformatics to decipher the ascorbic acid metabolic network in tomato.Plant molecular biology · 2016Article
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- Metabolic engineering of the fungal D-galacturonate pathway for L-ascorbic acid production.Microbial cell factories · 2015Article
- Impact of oxidative stress on ascorbate biosynthesis in Chlamydomonas via regulation of the VTC2 gene encoding a GDP-L-galactose phosphorylase.The Journal of biological chemistry · 2012Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously proposed that Euglena gracilis possesses a pathway for the production of ascorbate (AsA) through d-galacturonate/L-galactonate as representative intermediates ( Shigeoka, S., Nakano, Y., and Kitaoka, S. (1979) J. Nutr. Sci. Vitaminol. 25, 299-307 ). However, genetic evidence proving that the pathway exists has not been obtained yet. We report here the identification of a gene encoding aldonolactonase, which catalyzes a penultimate step of the biosynthesis of AsA in Euglena. By a BLAST search, we identified one candidate for the enzyme having significant sequence identity with rat gluconolactonase, a key enzyme for the production of AsA via d-glucuronate in animals. The purified recombinant aldonolactonase expressed in Escherichia coli catalyzed the reversible reaction of L-galactonate and L-galactono-1,4-lactone with zinc ion as a cofactor. The apparent K(m) values for L-galactonate and L-galactono-1,4-lactone were 1.55 +/- 0.3 and 1.67 +/- 0.39 mm, respectively. The cell growth of Euglena was arrested by silencing the expression of aldonolactonase through RNA interference and then restored to the normal state by supplementation with L-galactono-1,4-lactone. Euglena cells accumulated more AsA on supplementation with d-galacturonate than d-glucuronate. The present results indicate that aldonolactonase is significant for the biosynthesis of AsA in Euglena cells, which predominantly utilize the pathwayviad-galacturonate/L-galactonate. The identification of aldonolactonase provides the first insight into the biosynthesis of AsA via uronic acids as the intermediate in photosynthetic algae including Euglena.
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