ArticleMolecular biology reports2009
Dual role of shikonin in early and late stages of collagen type II arthritis.
Article in Molecular biology reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Immunopharmacological effects of shikonin in experimental rheumatoid arthritis: a systematic review and meta-analysis of in vivo studies.Naunyn-Schmiedeberg's archives of pharmacology · 2026Pooled it
- Pharmacological Significance ofFoods (Basel, Switzerland) · 2024Review
- Shikonin alleviates collagen-induced arthritis mice by inhibiting M1 macrophage polarization.Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2022Article
- Shikonin attenuates rheumatoid arthritis by targeting SOCS1/JAK/STAT signaling pathway of fibroblast like synoviocytes.Chinese medicine · 2021Article
- TlR2 and TlR4 are involved in the treatment of rheumatoid arthritis synovial fibroblasts with a medicated serum of asarinin through inhibition of TExperimental and therapeutic medicine · 2020Article
- Toll-Like Receptor 4-Myeloid Differentiation Primary Response Gene 88 Pathway Is Involved in the Shikonin Treatment of CIA by Regulating Treg/Th17 Expression.Evidence-based complementary and alternative medicine : eCAM · 2018Article
- Shikonin changes the lipopolysaccharide-induced expression of inflammation-related genes in macrophages.Journal of natural medicines · 2017Article
- Shikonin inhibits TNF-α production through suppressing PKC-NF-κB-dependent decrease of IL-10 in rheumatoid arthritis-like cell model.Journal of natural medicines · 2017Article
- Investigation of the effect of phlomisoside F on complete Freund's adjuvant-induced arthritis.Experimental and therapeutic medicine · 2017Article
- Shikonin Inhibits Inflammatory Response in Rheumatoid Arthritis Synovial Fibroblasts via lncRNA-NR024118.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Therapeutic effect of a hydroxynaphthoquinone fraction on dextran sulfate sodium-induced ulcerative colitis.World journal of gastroenterology · 2014Article
- Effectiveness of a hydroxynaphthoquinone fraction from Arnebia euchroma in rats with experimental colitis.World journal of gastroenterology · 2013Article
- Developing Phytocompounds from Medicinal Plants as Immunomodulators.Advances in botanical research · 2012Article
- Beneficial effect of shikonin on experimental colitis induced by dextran sulfate sodium in BALB/c mice.Evidence-based complementary and alternative medicine : eCAM · 2012Article
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo investigate the anti-inflammatory or immunomodulatory effect of shikonin on early stage and established murine collagen-induced arthritis (CIA).
methodsMouse were injected intraperitoneally with shikonin (5 mg/kg) for 10 days along before or after the onset of CIA. The arthritis response was monitored visually by macroscopic scoring. Reverse transcription-polymerase chain reaction and western blotting were employed to determine the mRNA and protein expression of cytokine in patella with adjacent synovium in CIA mouse. Histology of knee was used to assess the occurrence of cartilage destruction and bone erosion.
resultsShikonin (5 mg/kg) treatment along had no effect on macroscopic score and incidence of arthritis on early stage of CIA. However, a pronounced amelioration of macroscopic score and cartilage destruction was found in mouse treated with shikonin on established CIA for 10 days. Moreover, The mRNA levels of Th1 cytokines [tumor necrosis factor-alpha and interleukin (IL)-12] was significantly inhibited both in the synovial tissue and in the articular cartilage in treated groups compared with those in control groups, whereas the mRNA and protein levels of Th2 cytokines (IL-10 and IL-4) remained elevated throughout the treatment period. Moreover, the inflammatory cytokine, the mRNA and protein levels of IL-6 was down-regulated in mice with established CIA after treatment with shikonin. T-box expressed in T cells (T-bet) mRNA levels were decreased in shikonin compared with control group, and GATA-3 mRNA levels were higher than that in control group.
conclusionShikonin treatment on established CIA can inhibit Th1 cytokines expression and induce Th2 cytokines expression in mice with established CIA. The inhibited effect of shikonin on Th1 cytokines expression may be mediated not only by inhibiting Th1 responses through T-bet mechanism, but also by inducing anti-inflammatory mediators such as IL-10 and IL-4 through a GATA-3 dependent mechanism.
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