ArticleMolecular biology reports2009
Recent advances on the GAP promoter derived expression system of Pichia pastoris.
Article in Molecular biology reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
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- Toward food-grade production of the Glutamicibacter halophytocola diamine oxidase using Komagataella phaffii.AMB Express · 2025Article
- The MFα signal sequence in yeast-based protein secretion: challenges and innovations'.Applied microbiology and biotechnology · 2025Review
- Advancing recombinant protein expression in Komagataella phaffii: opportunities and challenges.FEMS yeast research · 2025Review
- Novel transcriptional regulation of the GAP promoter in Pichia pastoris towards high expression of heterologous proteins.Microbial cell factories · 2024Article
- Polyester degradation by soil bacteria: identification of conserved BHETase enzymes in Streptomyces.Communications biology · 2024Article
- Recent progress on heterologous protein production in methylotrophic yeast systems.World journal of microbiology & biotechnology · 2024Review
- Optimal fermentation conditions for growth and recombinant protein production inCurrent research in food science · 2024Article
- Optimization Workflow of Fumonisin Esterase Production for Biocatalytic Degradation of Fumonisin BLife (Basel, Switzerland) · 2023Article
- Industrial Production of Proteins withBiomolecules · 2023Review
- Engineering of Promoters for Gene Expression in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Improved Production ofCurrent issues in molecular biology · 2021Article
- Integrated Strategies for Enhancing the Expression of the AqCoA Chitosanase in Pichia pastoris by Combined Optimization of Molecular Chaperones Combinations and Copy Numbers via a Novel Plasmid pMC-GAP.Applied biochemistry and biotechnology · 2021Article
- Targeted Deletion of Los1 Homologue Affects the Production of a Recombinant Model Protein in Pichia pastoris.Iranian biomedical journal · 2021Article
- A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases.Molecules (Basel, Switzerland) · 2021Review
- Development of synthetic biology tools to engineerSynthetic and systems biotechnology · 2021Review
- Article
- Rationale-based selection of optimal operating strategies and gene dosage impact on recombinant protein production in Komagataella phaffii (Pichia pastoris).Microbial biotechnology · 2020Article
- High-Level Production of a Thermostable Mutant ofInternational journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pichia pastoris is an efficient host for the expression and secretion of heterologous proteins and the most important feature of P. pastoris is the existence of a strong and tightly regulated promoter from the alcohol oxidase I (AOX1) gene. The AOX1 promoter (pAOX1) has been used to express foreign genes and to produce a variety of recombinant proteins in P. pastoris. However, some efforts have been made to develop new alternative promoters to pAOX1 to avoid the use of methanol. The glyceraldehyde-3-phosphate dehydrogenase promoter (pGAP) has been used for constitutive expression of many heterologous proteins. The pGAP-based expression system is more suitable for large-scale production because the hazard and cost associated with the storage and delivery of large volume of methanol are eliminated. Some important developments and features of this expression system will be summarized in this review.
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18781398What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.