Evidence map›Paper›PMID 18781356›Full record

ArticleCurrent microbiology2008

Genes involved in the benzoate catabolic pathway in Acinetobacter calcoaceticus PHEA-2.

Yuhua Zhan, Haiying Yu, Yongliang Yan, Ming Chen, Wei Lu, Shuying Li, Zixin Peng, Wei Zhang, Shuzhen Ping, Jin Wang and 1 more

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Article in Current microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Yuhua ZhanCollege of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China.
Haiying Yu
Yongliang Yan
Ming Chen
Wei Lu
Shuying Li
Zixin Peng
Wei Zhang
Shuzhen Ping
Jin Wang
Min Lin
Chinese Academy of Agricultural Sciences · CNBiotechnology Research Institute · CNChina Agricultural University · CNSouthwest University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A putative benM gene encoding a LysR-type regulator located upstream from the benA gene was found in Acinetobacter calcoaceticus PHEA-2. Disruption of benM or benA destroyed the ability of PHEA-2 to utilize benzoate. The benM mutant was used to construct a genomic library for isolation of the complete gene cluster responsible for benzoate degradation. Sequence analysis showed that the cluster has three putative operons: benM, benABCDE, and benKP. Unlike many well-characterized benzoate-degrading bacteria, muconate is unable to induce in vivo transcription of the PHEA-2 ben cluster. Reverse transcriptase-polymerase chain reaction (RT-PCR) results showed that the benABCDE operon is activated by the BenM protein in the presence of benzoate. Moreover, a gel-retardation assay demonstrated that BenM binds to the promotor region of the benA gene. The activities of catechol 1,2-dioxygenase (C12O) and catechol 2,3-dioxygenase (C23O) showed that PHEA-2 converted benzoate to catechol for further degradation, possibly via an ortho-cleavage pathway.

Indexed as

Acinetobacter calcoaceticusBacterial ProteinsBenzoatesCatechol 1,2-DioxygenaseCatechol 2,3-DioxygenaseDNA, BacterialElectrophoretic Mobility Shift AssayGene DeletionGene Expression ProfilingGene OrderMetabolic Networks and PathwaysMolecular Sequence DataMultigene FamilyOperonPromoter Regions, GeneticProtein BindingBacterial ProteinsBenzoatesCatechol 1,2-DioxygenaseCatechol 2,3-DioxygenaseDNA, Bacterialmuconic acidSorbic Acid

Identifiers

PMID18781356
OpenAlexW2006103378

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.