ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2008
High resolution melting analysis of almond SNPs derived from ESTs.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Rapid and cost-effective screening of newly identified microsatellite loci by high-resolution melting analysis.Molecular genetics and genomics : MGG · 2011Pooled it
- Characteristics of Candida albicans Derived From HIV-Positive Individuals With Oral Candidiasis: Genotyping, Phenotypic Variation, Antifungal Susceptibility, and Biofilm Formation.Journal of clinical laboratory analysis · 2024Article
- Successful development of molecular diagnostic technology combining mini-barcoding and high-resolution melting for traditional Chinese medicine agarwood species based on single-nucleotide polymorphism in the chloroplast genome.Frontiers in plant science · 2024Article
- A recessive mutation in muscadine grapes causes berry color-loss without influencing anthocyanin pathway.Communications biology · 2022Article
- The Relevance of Discovering and Recovering the Biodiversity of Apulian Almond Germplasm by Means of Molecular and Phenotypic Markers.Plants (Basel, Switzerland) · 2022Article
- Sexing chickens (Gallus gallus domesticus) with high-resolution melting analysis using feather crude DNA.Poultry science · 2021Article
- Mining Grapevine Downy Mildew Susceptibility Genes: A Resource for Genomics-Based Breeding and Tailored Gene Editing.Biomolecules · 2021Article
- Integration of advanced technologies for plant variety and cultivar identification.Journal of biosciences · 2021Review
- Identification of the 'Haryejosaeng' mandarin cultivar by multiplex PCR-based SNP genotyping.Molecular biology reports · 2020Article
- Development of species-specific molecular markers in Vanilla for seedling selection of hybrids.Molecular biology reports · 2020Article
- Article
- Whole Genome Resequencing of Capsicum baccatum and Capsicum annuum to Discover Single Nucleotide Polymorphism Related to Powdery Mildew Resistance.Scientific reports · 2018Article
- SLAF-based high-density genetic map construction and QTL mapping for major economic traits in sea urchin Strongylocentrotus intermedius.Scientific reports · 2018Article
- Association Mapping Analysis for Fruit Quality Traits inFrontiers in plant science · 2018Article
- The potential of aerosol eDNA sampling for the characterisation of commercial seed lots.PloS one · 2018Article
- Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice.Frontiers in pharmacology · 2018Article
- Authenticity analyses of Rhizoma Paridis using barcoding coupled with high resolution melting (Bar-HRM) analysis to control its quality for medicinal plant product.Chinese medicine · 2018Article
- Applications of molecular markers in the discrimination ofJournal of ginseng research · 2017Review
- Comparison of traditional and new generation DNA markers declares high genetic diversity and differentiated population structure of wild almond species.Scientific reports · 2017Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High resolution melting curve (HRM) is a recent advance for the detection of SNPs. The technique measures temperature induced strand separation of short PCR amplicons, and is able to detect variation as small as one base difference between samples. It has been applied to the analysis and scan of mutations in the genes causing human diseases. In plant species, the use of this approach is limited. We applied HRM analysis to almond SNP discovery and genotyping based on the predicted SNP information derived from the almond and peach EST database. Putative SNPs were screened from almond and peach EST contigs by HRM analysis against 25 almond cultivars. All 4 classes of SNPs, INDELs and microsatellites were discriminated, and the HRM profiles of 17 amplicons were established. The PCR amplicons containing single, double and multiple SNPs produced distinctive HRM profiles. Additionally, different genotypes of INDEL and microsatellite variations were also characterised by HRM analysis. By sequencing the PCR products, 100 SNPs were validated/revealed in the HRM amplicons and their flanking regions. The results showed that the average frequency of SNPs was 1:114 bp in the genic regions, and transition to transversion ratio was 1.16:1. Rare allele frequencies of the SNPs varied from 0.02 to 0.5, and the polymorphic information contents of the SNPs were from 0.04 to 0.53 at an average of 0.31. HRM has been demonstrated to be a fast, low cost, and efficient approach for SNP discovery and genotyping, in particular, for species without much genomic information such as almond.
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