ArticleGenetics2008
Retention of induced mutations in a Drosophila reverse-genetic resource.
Article in Genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- EMS-induced genomic variation and QTL-seq analysis of safflower spininess through whole genome sequencing (WGS).BMC genomics · 2025Article
- Review
- Article
- Genomic variation between PRSV resistant transgenic SunUp and its progenitor cultivar Sunset.BMC genomics · 2020Article
- Transcriptome sequencing reveals hotspot mutation regions and dwarfing mechanisms in wheat mutants induced by γ-ray irradiation and EMS.Journal of radiation research · 2020Article
- Mutants forG3 (Bethesda, Md.) · 2017Article
- Whole-Genome Sequencing and iPLEX MassARRAY Genotyping Map an EMS-Induced Mutation Affecting Cell Competition in Drosophila melanogaster.G3 (Bethesda, Md.) · 2016Article
- Genomic Scars Generated by Polymerase Theta Reveal the Versatile Mechanism of Alternative End-Joining.PLoS genetics · 2016Article
- Rare recombination events generate sequence diversity among balancer chromosomes in Drosophila melanogaster.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Genome-wide survey of artificial mutations induced by ethyl methanesulfonate and gamma rays in tomato.Plant biotechnology journal · 2016Article
- The Genetic Map Enters Its Second Century.Genetics · 2015Article
- Large-scale identification of chemically induced mutations in Drosophila melanogaster.Genome research · 2014Article
- A genetic screen based on in vivo RNA imaging reveals centrosome-independent mechanisms for localizing gurken transcripts in Drosophila.G3 (Bethesda, Md.) · 2014Article
- Tango7 directs cellular remodeling by the Drosophila apoptosome.Genes & development · 2013Article
- Whole Genome Sequencing and a New Bioinformatics Platform Allow for Rapid Gene Identification in D. melanogaster EMS Screens.Biology · 2012Article
- Induction, rapid fixation and retention of mutations in vegetatively propagated banana.Plant biotechnology journal · 2012Article
- Targeting the motor regulator Klar to lipid droplets.BMC cell biology · 2011Article
- Molecular genetic analyses of polytene chromosome region 72A-D in Drosophila melanogaster reveal a gene desert in 72D.PloS one · 2011Article
- Rapid identification of heterozygous mutations in Drosophila melanogaster using genomic capture sequencing.Genome research · 2010Article
- Notch ligand activity is modulated by glycosphingolipid membrane composition in Drosophila melanogaster.The Journal of cell biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Targeting induced local lesions in genomes (TILLING) is a reverse-genetic method for identifying point mutations in chemically mutagenized populations. For functional genomics, it is ideal to have a stable collection of heavily mutagenized lines that can be screened over an extended period of time. However, long-term storage is impractical for Drosophila, so mutant strains must be maintained by continual propagation of live cultures. Here we evaluate a strategy in which ethylmethane sulfonate (EMS) mutagenized chromosomes were maintained as heterozygotes with balancer chromosomes for >100 generations before screening. The strategy yielded a spectrum of point mutations similar to those found in previous studies of EMS-induced mutations, as well as 2.4% indels (insertions and deletions). Our analysis of 1887 point mutations in 148 targets showed evidence for selection against deleterious lesions and differential retention of lesions among targets on the basis of their position relative to balancer breakpoints, leading to a broad distribution of mutational densities. Despite selection and differential retention, the success of a user-funded service based on screening a large collection several years after mutagenesis indicates sufficient stability for use as a long-term reverse-genetic resource. Our study has implications for the use of balancer chromosomes to maintain mutant lines and provides the first large-scale quantitative assessment of the limitations of using breeding populations for repositories of genetic variability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.