ArticleGenetics2008
Secondary mutations correct fitness defects in Toxoplasma gondii with dinitroaniline resistance mutations.
Article in Genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- TK216 targets microtubules in Ewing sarcoma cells.Cell chemical biology · 2022Article
- Unveiling the Possible Oryzalin-Binding Site in the α-Tubulin ofACS omega · 2022Article
- Inhibiting parasite proliferation using a rationally designed anti-tubulin agent.EMBO molecular medicine · 2021Article
- Investigating the presence of compensatory evolution in dicamba resistant IAA16 mutated kochia (Bassia scoparia)Pest management science · 2021Article
- Dinitroaniline Herbicide Resistance and Mechanisms in Weeds.Frontiers in plant science · 2021Review
- Auranofin Resistance inFrontiers in cellular and infection microbiology · 2021Article
- Drug Resistance inFrontiers in microbiology · 2018Review
- Article
- Targeting Toxoplasma tubules: tubulin, microtubules, and associated proteins in a human pathogen.Eukaryotic cell · 2015Review
- Evaluation of the growth-inhibitory effect of trifluralin analogues on in vitro cultured Babesia bovis parasites.International journal for parasitology. Drugs and drug resistance · 2013Article
- A SAS-6-like protein suggests that the Toxoplasma conoid complex evolved from flagellar components.Eukaryotic cell · 2013Article
- SPM1 stabilizes subpellicular microtubules in Toxoplasma gondii.Eukaryotic cell · 2012Article
- Article
- α-Tubulin mutations alter oryzalin affinity and microtubule assembly properties to confer dinitroaniline resistance.Eukaryotic cell · 2010Article
- Selection and phenotype characterization of potassium antimony tartrate-resistant populations of four New World Leishmania species.Parasitology research · 2010Article
- Compensation of fitness costs and reversibility of antibiotic resistance mutations.Antimicrobial agents and chemotherapy · 2010Article
- Dinitroaniline activity in Toxoplasma gondii expressing wild-type or mutant alpha-tubulin.Antimicrobial agents and chemotherapy · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Dinitroanilines (oryzalin, trifluralin, ethafluralin) disrupt microtubules in protozoa but not in vertebrate cells, causing selective death of intracellular Toxoplasma gondii parasites without affecting host cells. Parasites containing alpha1-tubulin point mutations are dinitroaniline resistant but show increased rates of aberrant replication relative to wild-type parasites. T. gondii parasites bearing the F52Y mutation were previously demonstrated to spontaneously acquire two intragenic mutations that decrease both resistance levels and replication defects. Parasites bearing the G142S mutation are largely dependent on oryzalin for viable growth in culture. We isolated 46 T. gondii lines that have suppressed microtubule defects associated with the G142S or the F52Y mutations by acquiring secondary mutations. These compensatory mutations were alpha1-tubulin pseudorevertants or extragenic suppressors (the majority alter the beta1-tubulin gene). Many secondary mutations were located in tubulin domains that suggest that they function by destabilizing microtubules. Most strikingly, we identified seven novel mutations that localize to an eight-amino-acid insert that stabilizes the alpha1-tubulin M loop, including one (P364R) that acts as a compensatory mutation in both F52Y and G142S lines. These lines have reduced dinitroaniline resistance but most perform better than parental lines in competition assays, indicating that there is a trade-off between resistance and replication fitness.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.