ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2008
Feasibility of the seed specific cruciferin C promoter in the self excision Cre/loxP strategy focused on generation of marker-free transgenic plants.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Spatio-temporal regulation of recombinase expression enables efficient autoexcision of selectable marker genes in soybean and maize.BMC plant biology · 2025Article
- Engineered Expression of Vip3A in Green Tissues as a Feasible Approach for the Control of Insect Pests in Maize.Insects · 2023Article
- Effect of marker-free transgenic Chlamydomonas on the control of Aedes mosquito population and on plankton.Parasites & vectors · 2023Article
- Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus.Plants (Basel, Switzerland) · 2020Review
- An Efficient Gene Excision System in Maize.Frontiers in plant science · 2020Article
- Heat-Shock-Induced Removal of Transgenes Using the Gene-Deletor System in Hybrid Aspen (Genes · 2018Article
- Generation of marker-free transgenic hexaploid wheat via an Agrobacterium-mediated co-transformation strategy in commercial Chinese wheat varieties.Plant biotechnology journal · 2017Article
- Level of tissue differentiation influences the activation of a heat-inducible flower-specific system for genetic containment in poplar (Populus tremula L.).Plant cell reports · 2016Article
- Selectable Marker Gene Removal and Expression of Transgene by Inducible Promoter Containing FFDDIranian journal of biotechnology · 2015Article
- The pollen- and embryo-specific Arabidopsis DLL promoter bears good potential for application in marker-free Cre/loxP self-excision strategy.Plant cell reports · 2015Article
- Excision of a selectable marker gene in transgenic banana using a Cre/lox system controlled by an embryo specific promoter.Plant molecular biology · 2013Article
- Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.Journal of biosciences · 2012Review
- Methods of cell purification: a critical juncture for laboratory research and translational science.Cells, tissues, organs · 2012Article
- Cre-mediated seed-specific transgene excision in tobacco.Plant molecular biology · 2010Article
- A novel mannose-based selection system for plant transformation using celery mannose-6-phosphate reductase gene.Plant cell reports · 2010Article
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4 authors.
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Abstract
This work is focused on the generation of selectable marker-free transgenic tobacco plants using the self excision Cre/loxP system that is controlled by a strong seed specific Arabidopsis cruciferin C (CRUC) promoter. It involves Agrobacterium-mediated transformation using a binary vector containing the gus reporter gene and one pair of the loxP sites flanking the cre recombinase and selectable nptII marker genes (floxed DNA). Surprisingly, an ectopic activation of CRUC resulting in partial excision of floxed DNA was observed during regeneration of transformed cells already in calli. The regenerated T(0) plants were chimeric, but no ongoing ectopic expression was observed in these one-year-long invitro maintained plants. The process of the nptII removal was expected in the seeds; however, none of the analysed T(0) transgenic lines generated whole progeny sensitive to kanamycin. Detailed analyses of progeny of selected T(0)-30 line showed that 10.2% GUS positive plants had completely removed nptII gene while the remaining 86.4% were still chimeras. Repeated activation of the cre gene in T(2) seeds resulted in increased rate of marker-free plants, whereas four out of ten analysed chimeric T(1) plants generated completely marker-free progenies. This work points out the feasibility as well as limits of the CRUC promoter in the Cre/loxP strategy.
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