ArticlePloS one2007
The 5' leader of the mRNA encoding the mouse neurotrophin receptor TrkB contains two internal ribosomal entry sites that are differentially regulated.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 9 citations in OpenAlex.
- RNA-binding proteins impacting on internal initiation of translation.International journal of molecular sciences · 2013Review
- Cell type specificity and structural determinants of IRES activity from the 5' leaders of different HIV-1 transcripts.Nucleic acids research · 2013Article
- Cryptic transcripts from a ubiquitous plasmid origin of replication confound tests for cis-regulatory function.Nucleic acids research · 2012Article
- Transcripts with in silico predicted RNA structure are enriched everywhere in the mouse brain.BMC genomics · 2012Article
- Reversible upregulation of tropomyosin-related kinase receptor B by geranylgeranoic acid in human neuroblastoma SH-SY5Y cells.Journal of neuro-oncology · 2011Article
- Retinoic acid stimulation of VEGF secretion from human endometrial stromal cells is mediated by production of reactive oxygen species.The Journal of physiology · 2011Article
- Retinoic acid is a cofactor for translational regulation of vascular endothelial growth factor in human endometrial stromal cells.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Identification of an intercistronic internal ribosome entry site in a Marek's disease virus immediate-early gene.Journal of virology · 2009Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A single internal ribosomal entry site (IRES) in conjunction with IRES transactivating factors (ITAFs) is sufficient to recruit the translational machinery to a eukaryotic mRNA independent of the cap structure. However, we demonstrate that the mouse TrkB mRNA contains two independent IRESes. The mouse TrkB mRNA consists of one of two 5' leaders (1428 nt and 448 nt), both of which include the common 3' exon (Ex2, 344 nt). Dicistronic RNA transfections and in vitro translation of monocistronic RNA demonstrated that both full-length 5' leaders, as well as Ex2, exhibit IRES activity indicating the IRES is located within Ex2. Additional analysis of the upstream sequences demonstrated that the first 260 nt of exon 1 (Ex1a) also contains an IRES. Dicistronic RNA transfections into SH-SY5Y cells showed the Ex1a IRES is constitutively active. However, the Ex2 IRES is only active in response to retinoic acid induced neural differentiation, a state which correlates with the synthesis of the ITAF polypyrimidine tract binding protein (PTB1). Correspondingly, addition or knock-down of PTB1 altered Ex2, but not Ex1a IRES activity in vitro and ex vivo, respectively. These results demonstrate that the two functionally independent IRESes within the mouse TrkB 5' leader are differentially regulated, in part by PTB1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.