ArticleExperimental & molecular medicine2008
Transient phosphorylation of tumor associated microtubule associated protein (TMAP)/cytoskeleton associated protein 2 (CKAP2) at Thr-596 during early phases of mitosis.
Article in Experimental & molecular medicine, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Identification of CKAP2 as a Potential Target for Prevention of Gastric Cancer Progression: A Multi-Omics Study.International journal of molecular sciences · 2025Article
- CKAP2 Regulated by TFDP1 Promotes Metastasis and Proliferation of Colorectal Cancer through Affecting the Tumor Microenvironment.Journal of microbiology and biotechnology · 2024Article
- Human CKAP2L shows a cell cycle-dependent expression pattern and exhibits microtubule-stabilizing properties.FEBS open bio · 2024Article
- The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules.eLife · 2022Article
- CKAP2 expression is associated with glioma tumor growth and acts as a prognostic factor in high‑grade glioma.Oncology reports · 2018Article
- Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks.Journal of visualized experiments : JoVE · 2018Article
- CKAP2 phosphorylation by CDK1/cyclinB1 is crucial for maintaining centrosome integrity.Experimental & molecular medicine · 2017Article
- CKAP2 (cytoskeleton-associated protein2) is a new prognostic marker in HER2-negative luminal type breast cancer.PloS one · 2017Article
- Clinical implications of proliferation activity in T1 or T2 male gastric cancer patients.Experimental & molecular medicine · 2015Article
- Chromatin CKAP2, a new proliferation marker, as independent prognostic indicator in breast cancer.PloS one · 2014Article
- CKAP2 ensures chromosomal stability by maintaining the integrity of microtubule nucleation sites.PloS one · 2013Article
- Condensed chromatin staining of CKAP2 as surrogate marker for mitotic figures.Journal of cancer research and clinical oncology · 2012Article
- Characterization of mitosis-specific phosphorylation of tumor-associated microtubule-associated protein.Experimental & molecular medicine · 2009Article
- The function of p27 KIP1 during tumor development.Experimental & molecular medicine · 2009Review
- Cdk1-cyclin B1-mediated phosphorylation of tumor-associated microtubule-associated protein/cytoskeleton-associated protein 2 in mitosis.The Journal of biological chemistry · 2009Article
- Regulation of microtubule growth rates and their impact on chromosomal instability.Cell cycle (Georgetown, Tex.)Review
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tumor associated microtubule associated protein (TMAP), also known as cytoskeleton associated protein 2 (CKAP2) is a mitotic spindle-associated protein whose expression is cell cycle-regulated and also frequently deregulated in cancer cells. Two monoclonal antibodies (mAbs) against TMAP/CKAP2 were produced: B-1-13 and D-12-3. Interestingly, the reactivity of mAb D-12-3 to TMAP/CKAP2 was markedly decreased specifically in mitotic cell lysate. The epitope mapping study showed that mAb D-12-3 recognizes the amino acid sequence between 569 and 625 and that phosphorylation at T596 completely abolishes the reactivity of the antibody, suggesting that the differential reactivity originates from the phosphorylation status at T596. Immunofluorescence staining showed that mAb D-12-3 fails to detect TMAP/CKAP2 in mitotic cells between prophase and metaphase, but the staining becomes evident again in anaphase, suggesting that phosphorylation at T596 occurs transiently during early phases of mitosis. These results suggest that the cellular functions of TMAP/CKAP2 might be regulated by timely phosphorylation and dephosphorylation during the course of mitosis.
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