ArticleThe Journal of cell biology2008
The mouth of a dense-core vesicle opens and closes in a concerted action regulated by calcium and amphiphysin.
Article in The Journal of cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Fusion pore dynamics of large secretory vesicles define a distinct mechanism of exocytosis.The Journal of cell biology · 2023Article
- High-speed imaging reveals the bimodal nature of dense core vesicle exocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Circulating non-coding RNAs as new biomarkers and novel therapeutic targets in colorectal cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2021Review
- Spatiotemporal organization and protein dynamics involved in regulated exocytosis of MMP-9 in breast cancer cells.The Journal of general physiology · 2019Article
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- The fusion pore, 60 years after the first cartoon.FEBS letters · 2018Review
- Local protein dynamics during microvesicle exocytosis in neuroendocrine cells.Molecular biology of the cell · 2018Article
- Imaging the recruitment and loss of proteins and lipids at single sites of calcium-triggered exocytosis.Molecular biology of the cell · 2016Article
- Resident CAPS on dense-core vesicles docks and primes vesicles for fusion.Molecular biology of the cell · 2016Article
- Exocytosis and endocytosis in neuroendocrine cells: inseparable membranes!Frontiers in endocrinology · 2013Review
- Label-free characterization of living human induced pluripotent stem cells by subcellular topographic imaging technique using full-field quantitative phase microscopy coupled with interference reflection microscopy.Biomedical optics express · 2012Article
- Intracellular curvature-generating proteins in cell-to-cell fusion.The Biochemical journal · 2011Article
- Regulation of fusion pore closure and compound exocytosis in neuroendocrine PC12 cells by SCAMP1.Traffic (Copenhagen, Denmark) · 2011Article
- Rapid endocytosis and vesicle recycling in neuroendocrine cells.Cellular and molecular neurobiology · 2010Review
- Polarized TIRFM reveals changes in plasma membrane topology before and during granule fusion.Cellular and molecular neurobiology · 2010Article
- Loose coupling between calcium channels and sites of exocytosis in chromaffin cells.The Journal of physiology · 2009Article
- Myosin 2 maintains an open exocytic fusion pore in secretory epithelial cells.Molecular biology of the cell · 2009Article
Corrections and comments
- Erratum issued
- Reprinted as
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Secretion of hormones and peptides by neuroendocrine cells occurs through fast and slow modes of vesicle fusion but the mechanics of these processes are not understood. We used interference reflection microscopy to monitor deformations of the membrane surface and found that both modes of fusion involve the tightly coupled dilation and constriction of the vesicle. The rate of opening is calcium dependent and occurs rapidly at concentrations <5 muM [corrected] The fast mode of fusion is blocked selectively by a truncation mutant of amphiphysin. Vesicles do not collapse when fusion is triggered by strontium, rather they remain locked open and membrane scission is blocked. In contrast, constriction of the vesicle opening continues when endocytosis is blocked by inhibiting the function of dynamin. Thus, fast and slow modes of fusion involve similar membrane deformations and vesicle closure can be uncoupled from membrane scission. Regulation of these processes by calcium and amphiphysin may provide a mechanism for controlling the release of vesicle contents.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.