ArticleAntimicrobial agents and chemotherapy2008
Development, stability, and molecular mechanisms of macrolide resistance in Campylobacter jejuni.
Article in Antimicrobial agents and chemotherapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 70 citations in OpenAlex.
- Virulence and Antimicrobial Resistance inAntibiotics (Basel, Switzerland) · 2026Review
- Increasing rates ofAntimicrobial agents and chemotherapy · 2025Article
- Recurrent Campylobacter jejuni Infections withMicrobiology spectrum · 2023Article
- Antimicrobial Resistance Profiles and Macrolide Resistance Mechanisms ofMicroorganisms · 2021Article
- Genomic Analysis and Antimicrobial Resistance ofFrontiers in microbiology · 2021Article
- Resistance to Antibiotics in Thermophilic Campylobacters.Frontiers in medicine · 2021Review
- Antimicrobial resistance patterns and molecular resistance markers of Campylobacter jejuni isolates from human diarrheal cases.PloS one · 2020Article
- EmergingAntimicrobial agents and chemotherapy · 2019Article
- Antimicrobial Resistance inMicrobiology spectrum · 2018Review
- Molecular Basis of Macrolide Resistance inBioMed research international · 2018Article
- Genetic Basis and Clonal Population Structure of Antibiotic Resistance inFrontiers in microbiology · 2018Article
- The Current State of Macrolide Resistance in Campylobacter spp.: Trends and Impacts of Resistance Mechanisms.Applied and environmental microbiology · 2017Review
- Ribosomal Mutations Conferring Macrolide Resistance in Legionella pneumophila.Antimicrobial agents and chemotherapy · 2017Article
- Phenotypic and Transcriptomic Responses ofFrontiers in microbiology · 2017Article
- Selection of Resistance to Clarithromycin in Mycobacterium abscessus Subspecies.Antimicrobial agents and chemotherapy · 2017Article
- The Risk of Some Veterinary Antimicrobial Agents on Public Health Associated with Antimicrobial Resistance and their Molecular Basis.Frontiers in microbiology · 2016Review
- Important Role of a Putative Lytic Transglycosylase Cj0843c in β-Lactam Resistance in Campylobacter jejuni.Frontiers in microbiology · 2015Article
- Identification of a novel G2073A mutation in 23S rRNA in amphenicol-selected mutants of Campylobacter jejuni.PloS one · 2014Article
- Resistance mechanisms in Campylobacter jejuni.Virulence · 2013Review
- Mutational and transcriptomic changes involved in the development of macrolide resistance in Campylobacter jejuni.Antimicrobial agents and chemotherapy · 2013Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Previous studies of macrolide resistance in Campylobacter were primarily focused on strains from various origins or used in vitro systems. In this study, we conducted both in vitro and in vivo experiments to examine the development, stability, and genetic basis of macrolide resistance in Campylobacter jejuni using erythromycin-resistant (Ery(r)) mutants derived from the same parent strain. Chickens inoculated with low-level Ery(r) mutants (MIC = 32 or 64 microg/ml) at 15 days old did not shed highly Ery(r) mutants (MIC > 512 microg/ml) after prolonged exposure to a low dose of tylosin. The low-level Ery resistance was not stable in vitro or in vivo in the absence of macrolide selection pressure. However, high-level Ery resistance displayed remarkable stability in vitro and in vivo. Ribosomal sequence analysis of 69 selected Ery(r) mutants showed that specific point mutations (A2074G or A2074C) occurred in all highly Ery(r) mutants. No mutations in ribosomal protein L4 were observed in any of the in vitro-selected Ery(r) mutants. However, three specific mutations in L4, G74D, G57D, and G57V, were widely found among in vivo-selected Ery(r) mutants. Insertion of three amino acids, TSH, at position 98 in ribosomal protein L22 was observed only in mutants selected in vitro. Inactivation of the CmeABC efflux pump dramatically reduced Ery MICs in Ery(r) mutants. Together, these findings suggest that multiple factors contribute to the emergence of highly Ery(r) Campylobacter in chicken, reveal resistance level-dependent stability of macrolide resistance in C. jejuni, and indicate that C. jejuni utilizes complex and different mechanisms to develop Ery resistance in vitro and in vivo.
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