ArticleTraffic (Copenhagen, Denmark)2008
Modulation of host cell endocytosis by the type III cytotoxin, Pseudomonas ExoS.
Article in Traffic (Copenhagen, Denmark), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
- Individual contributions of exotoxins S and T on internalizedInfection and immunity · 2026Article
- Interplay between T3SS effectors, ExoY activation, and cGMP signaling in Pseudomonas aeruginosa infection.Nature communications · 2025Article
- Evidence for intracellularJournal of bacteriology · 2024Review
- Intracellular replication ofmSphere · 2023Article
- Intracellular replication of Pseudomonas aeruginosa in epithelial cells requires suppression of the caspase-4 inflammasome.bioRxiv : the preprint server for biology · 2023Article
- Exotoxin S secreted by internalized Pseudomonas aeruginosa delays lytic host cell death.PLoS pathogens · 2022Article
- Contact lens-related corneal infection: Intrinsic resistance and its compromise.Progress in retinal and eye research · 2020Review
- Targeting ADP-ribosylation as an antimicrobial strategy.Biochemical pharmacology · 2019Review
- The Effects of IFN-λ on Epithelial Barrier Function Contribute to Klebsiella pneumoniae ST258 Pneumonia.American journal of respiratory cell and molecular biology · 2019Article
- The Impact of ExoS onmBio · 2018Article
- Epithelial cell lysates induce ExoS expression and secretion by Pseudomonas aeruginosa.FEMS microbiology letters · 2018Article
- Oncogenic role of rab escort protein 1 through EGFR and STAT3 pathway.Cell death & disease · 2017Article
- Innate Immune Signaling Activated by MDR Bacteria in the Airway.Physiological reviews · 2016Review
- Mechanisms of phagocytosis and host clearance of Pseudomonas aeruginosa.American journal of physiology. Lung cellular and molecular physiology · 2014Review
- In vitro assays to monitor the activity of Pseudomonas aeruginosa Type III secreted proteins.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Exoenzyme S ADP-ribosylates Rab5 effector sites to uncouple intracellular trafficking.Infection and immunity · 2014Article
- Regulation of Rab5 function during phagocytosis of live Pseudomonas aeruginosa in macrophages.Infection and immunity · 2013Article
- Pseudomonas aeruginosa utilizes the type III secreted toxin ExoS to avoid acidified compartments within epithelial cells.PloS one · 2013Article
- Translocon-independent intracellular replication by Pseudomonas aeruginosa requires the ADP-ribosylation domain of ExoS.Microbes and infection · 2012Article
- Examining the role of actin-plasma membrane association in Pseudomonas aeruginosa infection and type III secretion translocation in migratory T24 epithelial cells.Infection and immunity · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Pseudomonas aeruginosa ExoS is a bifunctional type III cytotoxin that possesses Rho GTPase-activating protein (RhoGAP) and ADP-ribosyltransferase (ADPr) activities. In the current study, the RhoGAP and ADPr activities of ExoS were tested for the ability to disrupt mammalian epithelial cell physiology. RhoGAP, but not ADPr, inhibited internalization/phagocytosis of bacteria, while ADPr, but not RhoGAP, inhibited vesicle trafficking, both general fluid-phase uptake and EGF-activated EGF receptor (EGFR) degradation. In ADPr-intoxicated cells, upon EGF activation, EGFR co-localized with clathrin-coated vesicles (CCV), which did not mature into Rab5-positive early endosomes. Constitutively, active Rab5 recruited EGFR from CCV to early endosomes. Consistent with the inhibition of Rab5 function by ADPr, several Rab proteins including Rab5 and 9, but not Rab4, were ADP ribosylated by ExoS. Thus, the two enzymatic activities of ExoS have different effects on epithelial cells with RhoGAP inhibiting bacterial internalization and ADPr interfering with CCV maturation. The ability ADPr to inhibit mammalian vesicle trafficking provides a new mechanism for bacterial toxin-mediated virulence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.