ArticleWorld journal of gastroenterology2008
Efflux pump gene hefA of Helicobacter pylori plays an important role in multidrug resistance.
Article in World journal of gastroenterology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Sequential changes of antibiotic resistances ofWorld journal of gastroenterology · 2025Article
- Population structure ofJournal of clinical microbiology · 2025Article
- Overcoming antibiotic-resistantWorld journal of gastroenterology · 2025Review
- Copper enhances tetracycline resistance via the efflux transporter CrdAB-CzcBA inFrontiers in medicine · 2025Article
- Review
- Molecular Mechanisms of Biofilm Formation inAntibiotics (Basel, Switzerland) · 2024Review
- Unraveling resistance mechanisms in combination therapy: A comprehensive review of recent advances and future directions.Heliyon · 2024Review
- Horizontal gene transfer facilitates the molecular reverse-evolution of antibiotic sensitivity in experimental populations of H. pylori.Nature ecology & evolution · 2024Article
- Antibiotic Resistance ofJournal of Korean medical science · 2024Review
- HME, NFE, and HAE-1 efflux pumps in Gram-negative bacteria: a comprehensive phylogenetic and ecological approach.ISME communications · 2024Article
- Study of the Effect of Baicalin fromInternational journal of molecular sciences · 2023Article
- Antibiotic resistance in Helicobacter pylori: From potential biomolecular mechanisms to clinical practice.Journal of clinical laboratory analysis · 2023Review
- Helicobacter pylori Infection, Its Laboratory Diagnosis, and Antimicrobial Resistance: a Perspective of Clinical Relevance.Clinical microbiology reviews · 2022Review
- FBPAII and rpoBC, the Two Novel Secreted Proteins Identified by the Proteomic Approach from a Comparative Study between Antibiotic-Sensitive and Antibiotic-Resistant Helicobacter pylori-Associated Gastritis Strains.Infection and immunity · 2021Article
- Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.Chemical reviews · 2021Review
- Next Generation Sequencing for the Prediction of the Antibiotic Resistance inAntibiotics (Basel, Switzerland) · 2021Review
- Fighting the Antibiotic Crisis: Flavonoids as Promising Antibacterial Drugs AgainstFrontiers in cellular and infection microbiology · 2021Review
- Next-Generation Sequencing of the Whole Bacterial Genome for Tracking Molecular Insight into the Broad-Spectrum Antimicrobial Resistance ofMicroorganisms · 2020Article
- Trends inMicrobial genomics · 2020Review
- Effective therapeutic regimens in two South Asian countries with high resistance to majorAntimicrobial resistance and infection control · 2019Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo determine whether efflux systems contribute to multidrug resistance of H pylori.
methodsA chloramphenicol-induced multidrug resistance model of six susceptible H pylori strains (5 isolates and H pylori NCTC11637) was developed. Multidrug-resistant (MDR) strains were selected and the minimal inhibitory concentration (MIC) of erythromycin, metronidazole, penicillin G, tetracycline, and ciprofloxacin in multidrug resistant strains and their parent strains was determined by agar dilution tests. The level of mRNA expression of hefA was assessed by fluorescence real-time quantitative PCR. A H pylori LZ1026 knockout mutant (delta H pylori LZ1026) for (putative) efflux protein was constructed by inserting the kanamycin resistance cassette from pEGFP-N2 into hefA, and its susceptibility profiles to 10 antibiotics were evaluated.
resultsThe MIC of six multidrug-resistant strains (including 5 clinical isolates and H pylori NCTC11637) increased significantly (>or=4-fold) compared with their parent strains. The expression level of hefA gene was significantly higher in the MDR strains than in their parent strains (P=0.033). A H pylori LZ1026 mutant was successfully constructed and the delta H pylori LZ1026 was more susceptible to four of the 10 antibiotics. All the 20 strains displayed transcripts for hefA that confirmed the in vitro expression of these genes.
conclusionThe efflux pump gene hefA plays an important role in multidrug resistance of H pylori.
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