Evidence map›Paper›PMID 18777088›Full record

ArticleMolecular and cellular biochemistry2009

Homocysteine effects classical pathway of GPCR down regulation: Galpha(q/11), Galpha(12/13), G(i/o).

T P Vacek, U Sen, N Tyagi, M Kumar, K S Moshal, J C Passmore, S C Tyagi

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

T P VacekDepartment of Physiology & Biophysics, School of Medicine, University of Louisville, Health Sciences Center, Louisville, KY 40202, USA.
U Sen
N Tyagi
M Kumar
K S Moshal
J C Passmore
S C Tyagi
University of Louisville · US

Funding

Reversing diabetic muscle myopathy by exercise and exosomesR01HL074185 · NHLBI · UNIVERSITY OF LOUISVILLE · PI TYAGI, SURESH C. · 2003 to 2018
$5.7M
Mechanism of Vascular Remodeling in HyperhomocysteinemiaR01HL071010 · NHLBI · UNIVERSITY OF MISSISSIPPI MEDICAL CENTER · PI TYAGI, SURESH C. · 2002 to 2010
$2.7M
Mechanisms of Cerebral Vascular RemodelingR01NS051568 · NINDS · UNIVERSITY OF LOUISVILLE · PI TYAGI, SURESH C. · 2007 to 2011
$1.6M
Implications of Endothelial-Myocyte Uncoupling in Cardiac ArrhythmiaR01HL088012 · NHLBI · UNIVERSITY OF LOUISVILLE · PI TYAGI, SURESH C. · 2007 to 2010
$1.5M
NHLBI NIH HHS HL-71010NHLBI NIH HHS HL-74185NHLBI NIH HHS HL-88012NHLBI NIH HHS R01 HL071010NHLBI NIH HHS R01 HL074185NHLBI NIH HHS R01 HL088012NINDS NIH HHS NS-51568NINDS NIH HHS R01 NS051568
6 · The paper itself

Abstract

G protein-coupled receptors (GPCRs) are known to modulate intracellular effectors involved in cardiac function. We recently reported homocysteine (Hcy)-induced ERK-phosphorylation was suppressed by pertussis toxin (PTX), which suggested the involvement of GPCRs in initiating signal transduction. An activated GPCR undergoes down regulation via a known mechanism involving ERK, GRK2, beta-arrestin1: ERK activity increases; GRK2 activity increases; beta-arrestin1 is degraded. We hypothesized that Hcy treatment leads to GPCR activation and down regulation. Microvascular endothelial cells were treated with Hcy. Expression of phospho-ERK1 and phospho-GRK2 was determined using Western blot, standardized to ERK1, GRK2, and beta-actin. Hcy was shown to dephosphorylate GRK2, thereby enhancing the activity. The results provided further evidence that Hcy acts as an agonist to activate GPCRs, followed by their down regulation. Hcy was also shown to decrease the content of the following G proteins and other proteins: beta-arrestin1, Galpha(q/11), Galpha(12/13), G(i/o).

Indexed as

AnimalsCells, CulturedDose-Response Relationship, DrugDown-RegulationEndothelial CellsExtracellular Signal-Regulated MAP KinasesG-Protein-Coupled Receptor Kinase 2GTP-Binding Protein alpha Subunits, G12-G13GTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11HomocysteineRatsReceptors, G-Protein-CoupledSignal TransductionExtracellular Signal-Regulated MAP KinasesG-Protein-Coupled Receptor Kinase 2Grk2 protein, ratGTP-Binding Protein alpha Subunits, G12-G13GTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11HomocysteineReceptors, G-Protein-Coupled

Identifiers

PMID18777088
PMCPMC2667933
OpenAlexW2161300785

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.