ArticleOncogene2008
Cyclin-dependent kinase 4/6 activity is a critical determinant of pre-replication complex assembly.
Article in Oncogene, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- RB1 loss triggers dependence on ESRRG in retinoblastoma.Science advances · 2022Article
- Review
- Therapeutic potential of CDK4/6 inhibitors in renal cell carcinoma.Nature reviews. Urology · 2022Review
- Cyclin-Dependent Kinase Inhibitors and Their Therapeutic Potential in Colorectal Cancer Treatment.Frontiers in pharmacology · 2021Review
- The E3 ubiquitin ligase TRIP12 participates in cell cycle progression and chromosome stability.Scientific reports · 2020Article
- Interactome analysis of gene expression profiles identifies CDC6 as a potential therapeutic target modified by miR-215-5p in hepatocellular carcinoma.International journal of medical sciences · 2020Article
- Wogonin Induces Apoptosis and Reverses Sunitinib Resistance of Renal Cell Carcinoma CellsFrontiers in pharmacology · 2020Article
- Berberine downregulates CDC6 and inhibits proliferation via targeting JAK-STAT3 signaling in keratinocytes.Cell death & disease · 2019Article
- Cyclooxygenase-2 Influences Response to Cotargeting of MEK and CDK4/6 in a Subpopulation of Pancreatic Cancers.Molecular cancer therapeutics · 2018Article
- Article
- CBX3 promotes colon cancer cell proliferation by CDK6 kinase-independent function during cell cycle.Oncotarget · 2017Article
- KMTase Set7/9 is a critical regulator of E2F1 activity upon genotoxic stress.Cell death and differentiation · 2014Article
- Transcriptional repression of histone deacetylase 3 by the histone demethylase KDM2A is coupled to tumorigenicity of lung cancer cells.The Journal of biological chemistry · 2014Article
- MFG-E8 activates proliferation of vascular smooth muscle cells via integrin signaling.Aging cell · 2012Article
- Elevated expression of CDK4 in lung cancer.Journal of translational medicine · 2011Article
- Unexpected reduction of skin tumorigenesis on expression of cyclin-dependent kinase 6 in mouse epidermis.The American journal of pathology · 2011Article
- Regulation of RB transcription in vivo by RB family members.Molecular and cellular biology · 2010Article
- Differential regulation of cyclin-dependent kinase 4 (CDK4) and CDK6, evidence that CDK4 might not be activated by CDK7, and design of a CDK6 activating mutation.Molecular and cellular biology · 2009Article
Corrections and comments
- Retraction · 2023-03-22Duplication of/in Image · · see also: https://pubpeer.com/publications/55668942242FBA7D62E95BEEA36F78
- Retracted
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Cyclin-dependent kinases (CDKs) are important in regulating cell cycle transitions, particularly in coordinating DNA replication. Although the role of CDK2 activity on the replication apparatus has been extensively studied, the role of CDK4/6 in DNA replication control is less understood. Through targeted inhibition of CDK4/6 activity, we demonstrate that CDK4/6 kinase activity promotes cdc6 and cdt1 expression, and pre-replication complex (pre-RC) assembly in cycling cells. Conversely, CDK2 inhibition had no effect on the pre-RC assembly. The inhibition of pre-RC assembly is dependent on a functional retinoblastoma (RB) protein, which mediates downstream effects. As such, CDK4/6 inhibition has minimal effect on the replication apparatus in the absence of RB. The requirement of CDK4/6 was further interrogated using cells lacking D-type cyclins, in which replication complexes form normally, and correspondingly CDK4/6 inhibition had no effect on cell cycle or replication control. However, in the absence of D-type cyclins, CDK2 inhibition resulted in the attenuation of cdc6 and cdt1 levels, suggesting overlapping roles for CDK4/6 and CDK2 in regulating replication protein activity. Finally, CDK4/6 inhibition prevented the accumulation of cdc6 and cdt1 as cells progressed from mitosis through the subsequent G(1). Combined, these studies indicate that CDK4/6 activity is important in regulating the expression of these critical mediators of DNA replication.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.