ArticleApplied and environmental microbiology2008
In situ detection of freshwater fungi in an alpine stream by new taxon-specific fluorescence in situ hybridization probes.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 58 citations in OpenAlex.
- Newly Designed Fluorescence In Situ Hybridization Probes Reveal Previously Unknown Endophytic Abilities of Tuber magnatum in Herbaceous Plants.Microbial ecology · 2025Article
- Association between Aquatic Micropollutant Dissipation and River Sediment Bacterial Communities.Environmental science & technology · 2020Article
- Bacterial Diversity Controls Transformation of Wastewater-Derived Organic Contaminants in River-Simulating Flumes.Environmental science & technology · 2020Article
- Direct Metatranscriptome RNA-seq and Multiplex RT-PCR Amplicon Sequencing on Nanopore MinION - Promising Strategies for Multiplex Identification of Viable Pathogens in Food.Frontiers in microbiology · 2020Article
- Chrysoviruses Inhabited Symbiotic Fungi of Lichens.Viruses · 2019Article
- Considerations and consequences of allowing DNA sequence data as types of fungal taxa.IMA fungus · 2018Article
- Basidiomycete yeasts in the cortex of ascomycete macrolichens.Science (New York, N.Y.) · 2016Article
- The ecology and biogeochemistry of stream biofilms.Nature reviews. Microbiology · 2016Review
- Diversity and Dynamics of Active Small Microbial Eukaryotes in the Anoxic Zone of a Freshwater Meromictic Lake (Pavin, France).Frontiers in microbiology · 2016Article
- Simultaneous specific in planta visualization of root-colonizing fungi using fluorescence in situ hybridization (FISH).Mycorrhiza · 2014Article
- Extracellular transmission of a DNA mycovirus and its use as a natural fungicide.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Molecular techniques for pathogen identification and fungus detection in the environment.IMA fungus · 2011Article
- Phylogenetic and morphological assessment of two new species of Amniculicola and their allies (Pleosporales).Persoonia · 2009Article
Corrections and comments
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Authors and funding
5 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
New rRNA-targeting oligonucleotide probes permitted the fluorescence in situ hybridization (FISH) identification of freshwater fungi in an Austrian second-order alpine stream. Based on computer-assisted comparative sequence analysis, nine taxon-specific probes were designed and evaluated by whole-fungus hybridizations. Oligonucleotide probe MY1574, specific for a wide range of Eumycota, and the genus (Tetracladium)-specific probe TCLAD1395, as well as the species-specific probes ALacumi1698 (Alatospora acuminata), TRIang322 (Tricladium angulatum), and Alongi340 (Anguillospora longissima), are targeted against 18S rRNA, whereas probes TmarchB10, TmarchC1_1, TmarchC1_2, and AlongiB16 are targeted against the 28S rRNA of Tetracladium marchalianum and Anguillospora longissima, respectively. After 2 weeks and 3 months of exposure of polyethylene slides in the stream, attached germinating conidia and growing hyphae of freshwater fungi were accessible for FISH. Growing hyphae and germinating conidia on leaves and in membrane cages were also visualized by the new FISH probes.
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