ArticleApplied and environmental microbiology2008
AbiV, a novel antiphage abortive infection mechanism on the chromosome of Lactococcus lactis subsp. cremoris MG1363.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Members of theMicrobial genomics · 2026Article
- Revisiting lactococcal abortive infection-like antiphage systems: diversity, structures, and mechanisms.FEMS microbiology reviews · 2026Review
- The presence of plasmids in bacterial hosts alters phage isolation and infectivity.ISME communications · 2022Article
- Systematic strategies for developing phage resistant Escherichia coli strains.Nature communications · 2022Article
- Exploring the diversity of bacteriophage specific to Oenococcus oeni and Lactobacillus spp and their role in wine production.Applied microbiology and biotechnology · 2021Review
- Genome Characterization of Nocturne116, NovelMicroorganisms · 2021Article
- Phage Therapy: What Have We Learned?Viruses · 2018Review
- Characterization of prophages of Lactococcus garvieae.Scientific reports · 2017Article
- Polar flagella rotation in Vibrio parahaemolyticus confers resistance to bacteriophage infection.Scientific reports · 2016Article
- Mutational Analysis of the Antitoxin in the Lactococcal Type III Toxin-Antitoxin System AbiQ.Applied and environmental microbiology · 2015Article
- Effect of bile on nisin-mediated antibacterial activity and the expression of nisin genes of Lactococcus lactis W8.Current microbiology · 2013Article
- Effect of the abortive infection mechanism and type III toxin/antitoxin system AbiQ on the lytic cycle of Lactococcus lactis phages.Journal of bacteriology · 2013Article
- Involvement of the major capsid protein and two early-expressed phage genes in the activity of the lactococcal abortive infection mechanism AbiT.Applied and environmental microbiology · 2012Article
- Bacteriophages and dairy fermentations.Bacteriophage · 2012Article
- Lactococcal 936-type phages and dairy fermentation problems: from detection to evolution and prevention.Frontiers in microbiology · 2012Article
- Lactococcal abortive infection protein AbiV interacts directly with the phage protein SaV and prevents translation of phage proteins.Applied and environmental microbiology · 2010Article
- Characterization of Lactococcus lactis phage 949 and comparison with other lactococcal phages.Applied and environmental microbiology · 2010Article
- Genome organization and characterization of the virulent lactococcal phage 1358 and its similarities to Listeria phages.Applied and environmental microbiology · 2010Article
- Activation and transfer of the chromosomal phage resistance mechanism AbiV in Lactococcus lactis.Applied and environmental microbiology · 2009Article
- Identification and characterization of the phage gene sav, involved in sensitivity to the lactococcal abortive infection mechanism AbiV.Applied and environmental microbiology · 2009Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insertional mutagenesis with pGhost9::ISS1 resulted in independent insertions in a 350-bp region of the chromosome of Lactococcus lactis subsp. cremoris MG1363 that conferred phage resistance to the integrants. The orientation and location of the insertions suggested that the phage resistance phenotype was caused by a chromosomal gene turned on by a promoter from the inserted construct. Reverse transcription-PCR analysis confirmed that there were higher levels of transcription of a downstream open reading frame (ORF) in the phage-resistant integrants than in the phage-sensitive strain L. lactis MG1363. This gene was also found to confer phage resistance to L. lactis MG1363 when it was cloned into an expression vector. A subsequent frameshift mutation in the ORF completely eliminated the phage resistance phenotype, confirming that the ORF was necessary for phage resistance. This ORF provided resistance against virulent lactococcal phages belonging to the 936 and c2 species with an efficiency of plaquing of 10(-4), but it did not protect against members of the P335 species. A high level of expression of the ORF did not affect the cellular growth rate. Assays for phage adsorption, DNA ejection, restriction/modification activity, plaque size, phage DNA replication, and cell survival showed that the ORF encoded an abortive infection (Abi) mechanism. Sequence analysis revealed a deduced protein consisting of 201 amino acids which, in its native state, probably forms a dimer in the cytosol. Similarity searches revealed no homology to other phage resistance mechanisms, and thus, this novel Abi mechanism was designated AbiV. The mode of action of AbiV is unknown, but the activity of AbiV prevented cleavage of the replicated phage DNA of 936-like phages.
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