ArticleMolecular cell2008
Inhibition of a transcriptional pause by RNA anchoring to RNA polymerase.
Article in Molecular cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Structural basis for control of bacterial RNA polymerase pausing by a riboswitch and its ligand.Nature structural & molecular biology · 2023Article
- Structural basis of transcriptional regulation by a nascent RNA element, HK022 putRNA.Nature communications · 2022Article
- Conjugation Operons in Gram-Positive Bacteria with and without Antitermination Systems.Microorganisms · 2022Article
- Transcription complexes as RNA chaperones.Transcription · 2021Article
- A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria.Nucleic acids research · 2021Article
- Natural RNA Polymerase Aptamers Regulate Transcription in E. coli.Molecular cell · 2017Article
- RNA secondary structures regulate three steps of Rho-dependent transcription termination within a bacterial mRNA leader.Nucleic acids research · 2017Article
- A Two-Way Street: Regulatory Interplay between RNA Polymerase and Nascent RNA Structure.Trends in biochemical sciences · 2016Review
- Bacteriophage lambda: Early pioneer and still relevant.Virology · 2015Review
- Distinct pathways of RNA polymerase regulation by a phage-encoded factor.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Unusually long-lived pause required for regulation of a Rho-dependent transcription terminator.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Newly discovered antiterminator RNAs in bacteriophage.Journal of bacteriology · 2011Article
- Termination and antitermination: RNA polymerase runs a stop sign.Nature reviews. Microbiology · 2011Review
- Ribozymes and riboswitches: modulation of RNA function by small molecules.Biochemistry · 2010Review
- A processive riboantiterminator seeks a switch to make biofilms.Molecular microbiology · 2010Article
- RNA polymerase: a nexus of gene regulation.Methods (San Diego, Calif.) · 2009Article
- DNA sequences in gal operon override transcription elongation blocks.Journal of molecular biology · 2008Article
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
We describe a mechanism by which nascent RNA inhibits transcriptional pausing. PutL RNA of bacteriophage HK022 suppresses transcription termination at downstream terminators and pausing within a nearby U-rich sequence. In vitro transcription and footprinting assays reveal that this pausing results from backtracking of RNA polymerase and that binding of nascent putL RNA to polymerase limits backtracking by restricting re-entry of the transcript into the RNA exit channel. The restriction is local and relaxes as the transcript elongates. Our results suggest that putL RNA binds to the surface of polymerase close to the RNA exit channel, a region that includes amino acid residues important for antitermination. Although binding is essential for antipausing and antitermination, these two activities of put differ: antipausing is limited to the immediate vicinity of the putL site, but antitermination is not. We propose that RNA anchoring to the elongation complex is a widespread mechanism of pause regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.