ArticleMolecular cell2008
Bypassing Sir2 and O-acetyl-ADP-ribose in transcriptional silencing.
Article in Molecular cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Physical interactions within the SIR heterochromatin complex potentiate inter-subunit communication and gene repression.Cell reports · 2026Article
- UvHOS3-mediated histone deacetylation is essential for virulence and negatively regulates ustilaginoidin biosynthesis in Ustilaginoidea virens.Molecular plant pathology · 2024Article
- Regulation of the heterochromatin spreading reaction byOpen biology · 2023Review
- Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium.Molecular biology of the cell · 2017Article
- Histone Deacetylases with Antagonistic Roles in Saccharomyces cerevisiae Heterochromatin Formation.Genetics · 2016Article
- Determinants of Sir2-Mediated, Silent Chromatin Cohesion.Molecular and cellular biology · 2016Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Sirtuins in epigenetic regulation.Chemical reviews · 2015Review
- Metabolism and epigenetics.Annual review of cell and developmental biology · 2015Review
- A lysine deacetylase Hos3 is targeted to the bud neck and involved in the spindle position checkpoint.Molecular biology of the cell · 2014Article
- Chromatin affinity-precipitation using a small metabolic molecule: its application to analysis of O-acetyl-ADP-ribose.Cellular and molecular life sciences : CMLS · 2012Article
- Structural basis for the role of the Sir3 AAA+ domain in silencing: interaction with Sir4 and unmethylated histone H3K79.Genes & development · 2011Article
- A dual role of H4K16 acetylation in the establishment of yeast silent chromatin.The EMBO journal · 2011Article
- Targeted sister chromatid cohesion by Sir2.PLoS genetics · 2011Article
- Function and metabolism of sirtuin metabolite O-acetyl-ADP-ribose.Biochimica et biophysica acta · 2010Review
- Rpd3-dependent boundary formation at telomeres by removal of Sir2 substrate.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Spatial epigenetic control of mono- and bistable gene expression.PLoS biology · 2010Article
- Silent information regulator 3: the Goldilocks of the silencing complex.Genes & development · 2010Review
- Reconstitution of heterochromatin-dependent transcriptional gene silencing.Molecular cell · 2009Article
- Silent chromatin at the middle and ends: lessons from yeasts.The EMBO journal · 2009Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The yeast Sir2/3/4 complex forms a heterochromatin-like structure that represses transcription. The proteins nucleate at silencers and spread distally, utilizing the Sir2 NAD(+)-dependent histone deacetylase activity and the affinity of Sir3/4 for deacetylated histone tails. A by-product of the Sir2 reaction, O-acetyl-ADP-ribose (OAADPr), is thought to aid spreading by binding one of the Sir proteins. We developed a protein chimera approach to reexamine the contributions of Sir2. We show that a Sir3 chimera-bearing Hos3, an unrelated NAD(+)-independent histone deacetylase, substitutes for Sir2 in silencing. Sir3-Hos3 operates within the Sir pathway, spreading while deacetylating histones. Moreover, the chimera represses HM loci in strains lacking all five OAADPr-producing deacetylases, indicating that OAADPr is not necessary for silencing. Repression by a Hos3 hybrid bearing the targeting motifs of Sir2 shows that targeting doesn't require the Sir2 reaction. Together, these data demonstrate that protein deacetylation is the only essential function of Sir2 in creating silenced chromatin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.