ArticleBioorganic & medicinal chemistry letters2008
Two-color labeling of temporally defined protein populations in mammalian cells.
Article in Bioorganic & medicinal chemistry letters, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 72 citations in OpenAlex.
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- Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts:Chemical reviews · 2025Review
- Protein translation rate determines neocortical neuron fate.Nature communications · 2024Article
- Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation.RNA (New York, N.Y.) · 2022Article
- An Optimized Synthesis of Fmoc-l-Homopropargylglycine-OH.The Journal of organic chemistry · 2022Article
- Coupling chemical biology and vibrational spectroscopy for studies of amyloids in vitro and in cells.Current opinion in chemical biology · 2021Review
- A Legionella pneumophila Kinase Phosphorylates the Hsp70 Chaperone Family to Inhibit Eukaryotic Protein Synthesis.Cell host & microbe · 2019Article
- Effect of Noncanonical Amino Acids on Protein-Carbohydrate Interactions: Structure, Dynamics, and Carbohydrate Affinity of a Lectin Engineered with Fluorinated Tryptophan Analogs.ACS chemical biology · 2018Article
- Exploring ribosome composition and newly synthesized proteins through proteomics and potential biomedical applications.Expert review of proteomics · 2017Review
- Visualizing in situ translational activity for identifying and sorting slow-growing archaeal-bacterial consortia.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Super-Resolution Imaging of Plasma Membrane Proteins with Click Chemistry.Frontiers in cell and developmental biology · 2016Article
- Simultaneous Site-Specific Dual Protein Labeling Using Protein Prenyltransferases.Bioconjugate chemistry · 2015Article
- Stress-induced inhibition of translation independently of eIF2α phosphorylation.Journal of cell science · 2015Article
- Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling.ACS chemical biology · 2015Article
- Article
- Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans.Nature protocols · 2014Article
- Acute synthesis of CPEB is required for plasticity of visual avoidance behavior in Xenopus.Cell reports · 2014Article
- Teaching old NCATs new tricks: using non-canonical amino acid tagging to study neuronal plasticity.Current opinion in chemical biology · 2013Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The proteome undergoes complex changes in response to disease, drug treatment, and normal cellular signaling processes. Characterization of such changes requires methods for time-resolved protein identification and imaging. Here, we describe the application of two reactive methionine (Met) analogues, azidohomoalanine (Aha) and homopropargylglycine (Hpg), to label two protein populations in fixed cells. Reactive lissamine rhodamine (LR), 7-dimethylaminocoumarin (DMAC), and bodipy-630 (BDPY) dyes were prepared and examined for use in selective dye-labeling of newly synthesized proteins in Rat-1 fibroblasts. The LR and DMAC, but not BDPY, fluorophores were found to enable selective, efficient labeling of subsets of the proteome; cells labeled with Aha and Hpg exhibited fluorescence emission three- to sevenfold more intense than that of control cells treated with Met. We also examined simultaneous and sequential pulse-labeling of cells with Aha and Hpg. After pulse-labeling, cells were treated with reactive LR and DMAC dyes, and labeled cells were imaged by fluorescence microscopy and analyzed by flow cytometry. The results of these studies demonstrate that amino acid labeling can be used to achieve selective two-color imaging of temporally defined protein populations in mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.