ArticleProceedings of the National Academy of Sciences of the United States of America2008
Development of input connections in neural cultures.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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65 citing papers in PubMed.
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- Modular architecture facilitates noise-driven control of synchrony in neuronal networks.Science advances · 2023Article
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- Strong connectivity in real directed networks.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Rich dynamics and functional organization on topographically designed neuronal networksiScience · 2022Article
- Spike Timing-Dependent Plasticity with Enhanced Long-Term Depression Leads to an Increase of Statistical Complexity.Entropy (Basel, Switzerland) · 2022Article
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- Discrete field potentials produced by coherent activation of spinal dorsal horn neurons.Experimental brain research · 2022Article
- Percolation in networks with local homeostatic plasticity.Nature communications · 2022Article
- Spontaneous Activity Predicts Survival of Developing Cortical Neurons.Frontiers in cell and developmental biology · 2022Article
- Explosive Synchronization-Based Brain Modulation Reduces Hypersensitivity in the Brain Network: A Computational Model Study.Frontiers in computational neuroscience · 2022Article
- Inferring Excitatory and Inhibitory Connections in Neuronal Networks.Entropy (Basel, Switzerland) · 2021Article
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- Parkinson's disease patient-specific neuronal networks carrying the LRRK2 G2019S mutation unveil early functional alterations that predate neurodegeneration.NPJ Parkinson's disease · 2021Article
5 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
We introduce an approach for the quantitative assessment of the connectivity in neuronal cultures, based on the statistical mechanics of percolation on a graph. This allows us to monitor the development of the culture and to see the emergence of connectivity in the network. The culture becomes fully connected at a time equivalent to the expected time of birth. The spontaneous bursting activity that characterizes cultures develops in parallel with the connectivity. The average number of inputs per neuron can be quantitatively determined in units of m(0), the number of activated inputs needed to excite the neuron. For m(0) approximately 15 we find that hippocampal neurons have on average approximately 60-120 inputs, whereas cortical neurons have approximately 75-150, depending on neuronal density. The ratio of excitatory to inhibitory neurons is determined by using the GABA(A) antagonist bicuculine. This ratio changes during development and reaches the final value at day 7-8, coinciding with the expected time of the GABA switch. For hippocampal cultures the inhibitory cells comprise approximately 30% of the neurons in the culture whereas for cortical cultures they are approximately 20%. Such detailed global information on the connectivity of networks in neuronal cultures is at present inaccessible by any electrophysiological or other technique.
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