ArticleThe American journal of pathology2008
Gene silencing of complement C5a receptor using siRNA for preventing ischemia/reperfusion injury.
Article in The American journal of pathology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 106 citations in OpenAlex.
- Effect of platelet-rich plasma on angiogenic and regenerative properties in patients with critical limb ischemia.Regenerative therapy · 2025Review
- Ischemia-Reperfusion Injury in Kidney Transplantation: Mechanisms and Potential Therapeutic Targets.International journal of molecular sciences · 2024Review
- The Complement System in Kidney Transplantation.Cells · 2023Review
- Innate immune modulation in transplantation: mechanisms, challenges, and opportunities.Frontiers in transplantation · 2023Review
- Gene targeting as a therapeutic avenue in diseases mediated by the complement alternative pathway.Immunological reviews · 2023Review
- Role of Complement System in Kidney Transplantation: Stepping From Animal Models to Clinical Application.Frontiers in immunology · 2022Review
- Complement-targeting therapeutics for ischemia-reperfusion injury in transplantation and the potential forFrontiers in immunology · 2022Review
- Role of C5aR1 and C5L2 Receptors in Ischemia-Reperfusion Injury.Journal of clinical medicine · 2021Article
- Renal Delivery of Pharmacologic Agents During Machine Perfusion to Prevent Ischaemia-Reperfusion Injury: From Murine Model to Clinical Trials.Frontiers in immunology · 2021Review
- The innate immune response to allotransplants: mechanisms and therapeutic potentials.Cellular & molecular immunology · 2019Review
- Significance of Complement System in Ischemic Stroke: A Comprehensive Review.Aging and disease · 2019Review
- Complement Inhibitor CRIg/FH Ameliorates Renal Ischemia Reperfusion Injury via Activation of PI3K/AKT Signaling.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Protective effects of the AKT activator SC79 on renal ischemia-reperfusion injury.International journal of clinical and experimental pathology · 2018Article
- Prevention of allograft rejection in heart transplantation through concurrent gene silencing of TLR and Kinase signaling pathways.Scientific reports · 2016Article
- Complement activation in the context of stem cells and tissue repair.World journal of stem cells · 2015Review
- The effect of nuclear factor of activated T-cells (NFAT) in kidney I/R mediated by C5a/C5aR.International journal of clinical and experimental medicine · 2015Article
- Complement cascade and kidney transplantation: The rediscovery of an ancient enemy.World journal of transplantation · 2014Review
- Mannan-binding lectin-associated serine protease 2 is critical for the development of renal ischemia reperfusion injury and mediates tissue injury in the absence of complement C4.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Dissecting the complement pathway in hepatic injury and regeneration with a novel protective strategy.The Journal of experimental medicine · 2014Article
- Synergic silencing of costimulatory molecules prevents cardiac allograft rejection.Journal of translational medicine · 2014Article
Corrections and comments
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Authors and funding
13 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ischemia/reperfusion (I/R) injury in organ transplantation significantly contributes to graft failure and is untreatable using current approaches. I/R injury is associated with activation of the complement system, leading to the release of anaphylatoxins, such as C5a, and the formation of the membrane attack complex. Here, we report a novel therapy for kidney I/R injury through silencing of the C5a receptor (C5aR) gene using siRNA. Mice were injected with 50 microg of C5aR siRNA 2 days before induction of ischemia. Renal ischemia was then induced through clamping of the renal vein and artery of the left kidney for 25 minutes. The therapeutic effects of siRNA on I/R were evaluated by assessment of renal function, histopathology, and inflammatory cytokines. siRNA targeting C5aR efficiently inhibited C5aR gene expression both in vitro and in vivo. Administering C5aR siRNA to mice preserved renal function from I/R injury, as evidenced by reduced levels of serum creatinine and blood urea nitrogen in the treated groups. Inhibition of C5aR also diminished in vivo production of the pro-inflammatory cytokine tumor necrosis factor-alpha and chemokines MIP-2 and KC, resulting in the reduction of neutrophils influx and cell necrosis in renal tissues. This study demonstrates that siRNA administration represents a novel approach to preventing renal I/R injury and may be used in a variety of clinical settings, including transplantation and acute tubular necrosis.
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