ArticleJournal of experimental botany2008
Absolute quantification of Medicago truncatula sucrose synthase isoforms and N-metabolism enzymes in symbiotic root nodules and the detection of novel nodule phosphoproteins by mass spectrometry.
Article in Journal of experimental botany, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Sucrose synthase dynamics and its potential role in heat stress tolerance in cereals.Frontiers in plant science · 2025Review
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- PANOMICS meets germplasm.Plant biotechnology journal · 2020Review
- A Proteomic View on the Role of Legume Symbiotic Interactions.Frontiers in plant science · 2017Review
- Drought Stress Causes a Reduction in the Biosynthesis of Ascorbic Acid in Soybean Plants.Frontiers in plant science · 2017Article
- Ascribing Functions to Genes: Journey Towards Genetic Improvement of Rice Via Functional Genomics.Current genomics · 2016Article
- Drought and Recovery: Independently Regulated Processes Highlighting the Importance of Protein Turnover Dynamics and Translational Regulation in Medicago truncatula.Molecular & cellular proteomics : MCP · 2016Article
- Article
- The quest for tolerant varieties: the importance of integrating "omics" techniques to phenotyping.Frontiers in plant science · 2015Review
- Advances in plant proteomics toward improvement of crop productivity and stress resistancex.Frontiers in plant science · 2015Review
- Discordance between protein and transcript levels detected by selected reaction monitoring.Plant signaling & behavior · 2015Article
- Proteomics and Metabolomics: Two Emerging Areas for Legume Improvement.Frontiers in plant science · 2015Review
- Selected reaction monitoring to determine protein abundance in Arabidopsis using the Arabidopsis proteotypic predictor.Plant physiology · 2014Article
- Application of selected reaction monitoring mass spectrometry to field-grown crop plants to allow dissection of the molecular mechanisms of abiotic stress tolerance.Frontiers in plant science · 2013Article
- Medicago PhosphoProtein Database: a repository for Medicago truncatula phosphoprotein data.Frontiers in plant science · 2012Article
- Article
- Lesson from the stoichiometry determination of the cohesin complex: a short protease mediated elution increases the recovery from cross-linked antibody-conjugated beads.Journal of proteome research · 2011Article
- Knockdown of the symbiotic sucrose synthase MtSucS1 affects arbuscule maturation and maintenance in mycorrhizal roots of Medicago truncatula.Plant physiology · 2010Article
- Large-scale phosphoprotein analysis in Medicago truncatula roots provides insight into in vivo kinase activity in legumes.Plant physiology · 2010Article
- Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mass spectrometry (MS) has become increasingly important for tissue specific protein quantification at the isoform level, as well as for the analysis of protein post-translational regulation mechanisms and turnover rates. Thanks to the development of high accuracy mass spectrometers, peptide sequencing without prior knowledge of the amino acid sequence--de novo sequencing--can be performed. In this work, absolute quantification of a set of key enzymes involved in carbon and nitrogen metabolism in Medicago truncatula 'Jemalong A17' root nodules is presented. Among them, sucrose synthase (SuSy; EC 2.4.1.13), one of the central enzymes in sucrose cleavage in root nodules, has been further characterized and the relative phosphorylation state of the three most abundant isoforms has been quantified. De novo sequencing provided sequence information of a so far unidentified peptide, most probably belonging to SuSy2, the second most abundant isoform in M. truncatula root nodules. TiO(2)-phosphopeptide enrichment led to the identification of not only a phosphorylation site at Ser11 in SuSy1, but also of several novel phosphorylation sites present in other root nodule proteins such as alkaline invertase (AI; EC 3.2.1.26) and an RNA-binding protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.