ArticleJournal of neuroscience methods2008
Lipopolysaccharide-induced increases in cytokines in discrete mouse brain regions are detectable using Luminex xMAP technology.
Article in Journal of neuroscience methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 60 citations in OpenAlex.
- Selective increase of cerebrospinal fluid IL-6 during experimental systemic inflammation in humans: association with depressive symptoms.Molecular psychiatry · 2017Trial
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- Partial depletion of circulating neutrophil granulocytes in mice exacerbates the inflammatory response and hypothermia during LPS induced severe systemic inflammation.Frontiers in immunology · 2025Article
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- Disruption of the NMDA receptor GluN2A subunit abolishes inflammation-induced depression.Behavioural brain research · 2019Article
- GM-CSF Promotes Chronic Disability in Experimental Autoimmune Encephalomyelitis by Altering the Composition of Central Nervous System-Infiltrating Cells, but Is Dispensable for Disease Induction.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- TLR4 response mediates ethanol-induced neurodevelopment alterations in a model of fetal alcohol spectrum disorders.Journal of neuroinflammation · 2017Article
- Sex and genetic differences in the effects of acute diesel exhaust exposure on inflammation and oxidative stress in mouse brain.Toxicology · 2016Article
- Minocycline attenuates post-operative cognitive impairment in aged mice by inhibiting microglia activation.Journal of cellular and molecular medicine · 2016Article
- Brain-derived neurotrophic and immunologic factors: beneficial effects of riboflavin on motor disability in murine model of multiple sclerosis.Iranian journal of basic medical sciences · 2016Article
- A Window on the Study of Aversive Instrumental Learning: Strains, Performance, Neuroendocrine, and Immunologic Systems.Frontiers in behavioral neuroscience · 2016Article
- Impaired Spatial Learning Memory after Isoflurane Anesthesia or Appendectomy in Aged Mice is Associated with Microglia Activation.Journal of cell death · 2015Article
- Decreased Splenic CD4(+) T-Lymphocytes in Apolipoprotein M Gene Deficient Mice.BioMed research international · 2015Article
- Modulation of learning and memory by cytokines: signaling mechanisms and long term consequences.Neurobiology of learning and memory · 2014Review
- Impairment of autophagy in the central nervous system during lipopolysaccharide-induced inflammatory stress in mice.Molecular brain · 2014Article
- Greatly improved survival and neuroprotection in aquaporin-4-knockout mice following global cerebral ischemia.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Early activation of STAT3 regulates reactive astrogliosis induced by diverse forms of neurotoxicity.PloS one · 2014Article
- The in vivo therapeutic efficacy of the oncolytic adenovirus Delta24-RGD is mediated by tumor-specific immunity.PloS one · 2014Article
- Maternal immune activation causes age- and region-specific changes in brain cytokines in offspring throughout development.Brain, behavior, and immunity · 2013Article
- Sepsis-induced morbidity in mice: effects on body temperature, body weight, cage activity, social behavior and cytokines in brain.Psychoneuroendocrinology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Methods to determine cytokine protein content in samples of interest, such as enzyme-linked immunosorbent assay (ELISA), are often labor-intensive and costly. Furthermore, because ELISA requires relatively large sample volumes and protein concentrations, it is difficult using this technique to determine protein content for multiple cytokines from individual samples. Recently, Luminex has developed an open source hardware platform combining flow cytometry- and bead-based antibody capture that is capable of detecting multiple analytes from a single sample. In the present study we employed the Luminex 200 platform to determine the cytokine protein content in discrete brain regions of C57BL/6J mice. In spike-and-recovery experiments, known concentrations of murine recombinant interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)alpha were added either singly or as a mixture of all three to whole brain homogenates containing known quantities of total protein. Spiked samples were assayed for either a single cytokine or for multiple cytokines using 1-plex or 3-plex assay kits, respectively. In whole mouse brain homogenate we recovered between 81% and 103% of the recombinant cytokines. We then injected C57BL/6J mice intraperitoneally with bacterial lipopolysaccharide (LPS) and sacrificed them 4h later. We detected in samples taken from LPS-stimulated mice 4- to 870-fold increases in serum or spleen cytokine protein, and 1.5- to 16-fold increases in cytokine protein in discrete brain regions, relative to protein content in samples obtained from vehicle-treated animals. These results indicate that multiple cytokines may be reliably assayed from discrete regions of mouse brain using a single sample.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.