ArticleZoonoses and public health2009
The studies on the aetiology of diarrhoea in neonatal calves and determination of virulence gene markers of Escherichia coli strains by multiplex PCR.
Article in Zoonoses and public health, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
- Molecular epidemiology of group A rotavirus (RVA) and bovine coronavirus (BCoV) associated with neonatal calf diarrhea in Türkiye.Tropical animal health and production · 2026Article
- Detection of virulence factors ofOpen veterinary journal · 2025Article
- Development of a "Rapid-Crypto Colorimetric LAMP Test" to Detect Cryptosporidiosis in Feces of Newborns Calves.Acta parasitologica · 2024Article
- Prevalence of antimicrobial resistance in fecalFrontiers in microbiology · 2023Article
- Isolation of bacterial consortia with probiotic potential from the rumen of tropical calves.Journal of animal physiology and animal nutrition · 2023Article
- Neopterin, procalcitonin, clinical biochemistry, and hematology in calves with neonatal sepsis.Tropical animal health and production · 2021Article
- Molecular characterization of pathogenic Escherichia coli isolated from diarrheic and in-contact cattle and buffalo calves.Tropical animal health and production · 2020Article
- Age related differences in phylogenetic diversity, prevalence of Shiga toxins, Intimin, Hemolysin genes and select serogroups of Escherichia. coli from pastured meat goats detected in a longitudinal cohort study.BMC veterinary research · 2020Article
- A novel multiplex PCR-electronic microarray assay for rapid and simultaneous detection of bovine respiratory and enteric pathogens.Journal of virological methods · 2018Article
- Evaluation of the in-field efficacy of oregano essential oil administration on the control of neonatal diarrhea syndrome in calves.Research in veterinary science · 2017Article
- Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99.Scientific reports · 2017Article
- Evaluation of a protocol to reduce the incidence of neonatal calf diarrhoea on dairy herds.Preventive veterinary medicine · 2015Article
- Longitudinal study of Salmonella spp., diarrheagenic Escherichia coli, Rotavirus, and Coronavirus isolated from healthy and diarrheic calves in a Brazilian dairy herd.Tropical animal health and production · 2015Article
- Advances in prevention and therapy of neonatal dairy calf diarrhoea: a systematical review with emphasis on colostrum management and fluid therapy.Acta veterinaria Scandinavica · 2014Review
- Prevalence of rotavirus (GARV) and coronavirus (BCoV) associated with neonatal diarrhea in calves in western Algeria.Asian Pacific journal of tropical biomedicine · 2014Article
- Identification of virulence factors by multiplex PCR in Escherichia coli isolated from calves in Minas Gerais, Brazil.Tropical animal health and production · 2012Article
- Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam.Journal of veterinary science · 2011Article
- Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina.Revista Argentina de microbiologiaArticle
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of this study was to determine aetiological agents of diarrhoea in neonatal calves and to investigate virulence gene markers of Escherichia coli strains isolated from calves by multiplex polymerase chain reaction (PCR). Eighty-two diarrhoeic calves and 18 healthy calves were used as subjects. Faeces were taken from the rectums of all the calves and were subjected to bacterial culture. Antigen enzyme-linked immunosorbent assay (ELISA) was performed to detect rotavirus, coronavirus and E. coli K99 in faeces of all the calves. A multiplex PCR was used to characterize E. coli strains in all the calves. Escherichia coli was isolated from 37 faeces samples, Enterococcus ssp. was isolated from 22 faeces samples and Salmonella was isolated from one faeces sample in diarrhoeic calves. Furthermore, only E. coli was isolated from all 18 faeces samples of healthy calves. Of the 37 E. coli isolated from diarrhoeic calves, K99 (18.9%), F41 (18.9%), heat-stable enterotoxin a (STa) (18.9%), Shiga toxin 1 (Stx1; 13.5%) and Shiga toxin 2 (Stx2; 5.4%) and intimin (8.1%) genes were identified by multiplex PCR. Of the 18 E. coli isolated from healthy calves, K99 (16.6%) and intimin (55.5%) genes were identified by PCR. A total of 15 rotavirus, 11 coronavirus and 11 E. coli K99 were detected in diarrhoeic calves by the antigen ELISA. As a result, this study shows that rotavirus, coronavirus, E. coli and Enterococcus ssp. were determined to play a role in the aetiology of diarrhoea in the neonatal calves. K99, F41, STa, Stx1 and Stx2 were found as the most common virulence gene markers of E. coli strains isolated from calves with diarrhoea. Multiplex PCR may be useful for characterization of E. coli isolated from calves.
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