ArticleBiochemistry2008
Intrinsic ssDNA annealing activity in the C-terminal region of WRN.
Article in Biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Identification of 2-Sulfonyl/Sulfonamide Pyrimidines as Covalent Inhibitors of WRN Using a Multiplexed High-Throughput Screening Assay.Biochemistry · 2023Article
- Human RecQ Helicases in DNA Double-Strand Break Repair.Frontiers in cell and developmental biology · 2021Review
- Acetylation of Werner protein at K1127 and K1117 is important for nuclear trafficking and DNA repair.DNA repair · 2019Article
- Replication of G Quadruplex DNA.Genes · 2019Review
- Multiple RPAs make WRN syndrome protein a superhelicase.Nucleic acids research · 2018Article
- Werner syndrome: Clinical features, pathogenesis and potential therapeutic interventions.Ageing research reviews · 2017Review
- A Monomer of Pif1 Unwinds Double-Stranded DNA and It Is Regulated by the Nature of the Non-Translocating Strand at the 3'-End.Journal of molecular biology · 2016Article
- RECQL5 has unique strand annealing properties relative to the other human RecQ helicase proteins.DNA repair · 2016Article
- RAPADILINO RECQL4 mutant protein lacks helicase and ATPase activity.Biochimica et biophysica acta · 2012Article
- Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structures.Nucleic acids research · 2012Article
- DNA binding residues in the RQC domain of Werner protein are critical for its catalytic activities.Aging · 2012Article
- Superfamily 2 helicases.Frontiers in bioscience (Landmark edition) · 2012Review
- Unwinding and rewinding: double faces of helicase?Journal of nucleic acids · 2012Article
- The DNA repair endonuclease XPG interacts directly and functionally with the WRN helicase defective in Werner syndrome.Cell cycle (Georgetown, Tex.) · 2011Article
- A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation.Nucleic acids research · 2011Article
- WRN mutations in Werner syndrome patients: genomic rearrangements, unusual intronic mutations and ethnic-specific alterations.Human genetics · 2010Article
- An essential DNA strand-exchange activity is conserved in the divergent N-termini of BLM orthologs.The EMBO journal · 2010Article
- Distinct roles of RECQ1 in the maintenance of genomic stability.DNA repair · 2010Review
- Drosophila RecQ4 has a 3'-5' DNA helicase activity that is essential for viability.The Journal of biological chemistry · 2009Article
- Biochemical characterization of AtRECQ3 reveals significant differences relative to other RecQ helicases.Plant physiology · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Werner syndrome (WS) is a rare autosomal recessive disorder in humans characterized by premature aging and genetic instability. WS is caused by mutations in the WRN gene, which encodes a member of the RecQ family of DNA helicases. Cellular and biochemical studies suggest that WRN plays roles in DNA replication, DNA repair, telomere maintenance, and homologous recombination and that WRN has multiple enzymatic activities including 3' to 5' exonuclease, 3' to 5' helicase, and ssDNA annealing. The goal of this study was to map and further characterize the ssDNA annealing activity of WRN. Enzymatic studies using truncated forms of WRN identified a C-terminal 79 amino acid region between the RQC and the HRDC domains (aa1072-1150) that is required for ssDNA annealing activity. Deletion of the region reduced or eliminated ssDNA annealing activity of the WRN protein. Furthermore, the activity appears to correlate with DNA binding and oligomerization status of the protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.