ArticleCurrent protocols in microbiology2008
Laboratory maintenance of Ehrlichia chaffeensis and Ehrlichia canis and recovery of organisms for molecular biology and proteomics studies.
Article in Current protocols in microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Prolonged immune response to tick-borne Ehrlichia chaffeensis infection using a genetically modified live vaccine.Vaccine · 2025Article
- Article
- Whole Genome Sequencing and Comparative Analysis of the FirstMicroorganisms · 2024Article
- Modulation of Macrophage Redox and Apoptotic Processes toPathogens (Basel, Switzerland) · 2023Article
- MultipleFrontiers in cellular and infection microbiology · 2023Article
- Evaluating EcxR for Its Possible Role inInternational journal of molecular sciences · 2022Article
- Proteome analysis ofFrontiers in cellular and infection microbiology · 2022Article
- Mutations in Ehrlichia chaffeensis Genes ECH_0660 and ECH_0665 Cause Transcriptional Changes in Response to Zinc or Iron Limitation.Journal of bacteriology · 2021Article
- Integrated into Environmental BiofilmMicroorganisms · 2020Article
- Multiple Ehrlichia chaffeensis Genes Critical for Its Persistent Infection in a Vertebrate Host Are Identified by Random Mutagenesis Coupled withInfection and immunity · 2020Article
- Proteome Analysis Revealed Changes in Protein Expression Patterns Caused by Mutations inFrontiers in cellular and infection microbiology · 2019Article
- Protein and DNA synthesis demonstrated in cell-free Ehrlichia chaffeensis organisms in axenic medium.Scientific reports · 2018Article
- Impact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns.Scientific reports · 2018Article
- Antigen-Specific CD4Frontiers in immunology · 2018Article
- Protein aggregation in Ehrlichia chaffeensis during infection of mammalian cells.FEMS microbiology letters · 2017Article
- Amblyomma americanum ticks infected with in vitro cultured wild-type and mutants of Ehrlichia chaffeensis are competent to produce infection in naïve deer and dogs.Ticks and tick-borne diseases · 2017Article
- Article
- Attenuated Mutants of Ehrlichia chaffeensis Induce Protection against Wild-Type Infection Challenge in the Reservoir Host and in an Incidental Host.Infection and immunity · 2015Article
- Mutations in Ehrlichia chaffeensis Causing Polar Effects in Gene Expression and Differential Host Specificities.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Tick-borne illnesses are emerging as a major concern for human health in recent years. These include the human monocytic ehrlichiosis caused by the Amblyomma americanum tick-transmitted bacterium, Ehrlichia chaffeensis; human ewingii ehrlichiosis caused by Ehrlichia ewingii (also transmitted by A. americanum ticks); and human granulocytic anaplasmosis caused by the Ixodes scapularis tick-transmitted pathogen, Anaplasma phagocytophilum. Likewise, tick-borne rickettsial pathogens are also a major concern to the health of various vertebrates including dogs, cattle, and several wild animals. In vitro-cultured pathogens grown in a vertebrate host cell and a tick cell culture system will be useful in studies to understand the pathogenic differences as well as to perform experimental infection studies and to generate large quantities of purified antigens. In this unit, methods for culturing E. chaffeensis and Ehrlichia canis (a canine monocytic ehrlichiosis pathogen) in cell lines to represent vertebrate and tick hosts are described. The unit also includes methods useful in purifying bacteria from the host cells and to evaluate proteins by 2-D gel electrophoresis and western blotting.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.