ArticleJournal of immunology (Baltimore, Md. : 1950)2008
Functionally competent eosinophils differentiated ex vivo in high purity from normal mouse bone marrow.
Article in Journal of immunology (Baltimore, Md. : 1950), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 162 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
162 citing papers in PubMed, 241 citations in OpenAlex.
- Activation and Cell Death of Mouse Eosinophils in Response to Different Microenvironmental Stimuli.Cells · 2026Article
- GPR43 in eosinophils suppresses the emergence of pathogenic Siglec-FNature communications · 2025Article
- Eosinophil CD48 interactions with Candida albicans Als6 is protective in vitro and in mouse systemic candidiasis.Nature communications · 2025Article
- The role of eosinophils and their activation state in hypereosinophilia-associated heart disease.Frontiers in immunology · 2025Article
- The AHR repressor limits expression of antimicrobial genes but not AHR-dependent genes in intestinal eosinophils.Journal of leukocyte biology · 2024Article
- Macrophage-derived chemokine CCL22 establishes local LN-mediated adaptive thermogenesis and energy expenditure.Science advances · 2024Article
- CCR3-dependent eosinophil recruitment is regulated by sialyltransferase ST3Gal-IV.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Eosinophils promote CD8Signal transduction and targeted therapy · 2024Article
- Article
- 4-Octyl Itaconate Alleviates Airway Eosinophilic Inflammation by Suppressing Chemokines and Eosinophil Development.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Eosinophil Phenotypes Are Functionally Regulated by Resolvin D2 during Allergic Lung Inflammation.American journal of respiratory cell and molecular biology · 2023Article
- Fluoxetine restrains allergic inflammation by targeting an FcɛRI-ATP positive feedback loop in mast cells.Science signaling · 2023Article
- Eosinophils protect pressure overload- and β-adrenoreceptor agonist-induced cardiac hypertrophy.Cardiovascular research · 2023Article
- GATA1 controls numbers of hematopoietic progenitors and their response to autoimmune neuroinflammation.Blood advances · 2022Article
- Schistosomiasis Mansoni-Recruited Eosinophils: An Overview in the Granuloma Context.Microorganisms · 2022Review
- Treatment of allergic eosinophilic asthma through engineered IL-5-anchored chimeric antigen receptor T cells.Cell discovery · 2022Article
- Potent CCR3 Receptor Antagonist, SB328437, Suppresses Colonic Eosinophil Chemotaxis and Inflammation in theInternational journal of molecular sciences · 2022Article
- The aryl hydrocarbon receptor contributes to tissue adaptation of intestinal eosinophils in mice.The Journal of experimental medicine · 2022Article
- Eosinophils are an essential element of a type 2 immune axis that controls thymus regeneration.Science immunology · 2022Article
- Siglec-F Promotes IL-33-Induced Cytokine Release from Bone Marrow-Derived Eosinophils Independently of the ITIM and ITIM-like Motif Phosphorylation.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
102 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We have devised an ex vivo culture system which generates large numbers of eosinophils at high purity (>90%) from unselected mouse bone marrow progenitors. In response to 4 days of culture with recombinant mouse FLT3-L and recombinant mouse stem cell factor followed by recombinant mouse IL-5 alone thereafter, the resulting bone marrow-derived eosinophils (bmEos) express immunoreactive major basic protein, Siglec F, IL-5R alpha-chain, and transcripts encoding mouse eosinophil peroxidase, CCR3, the IL-3/IL-5/GM-CSF receptor common beta-chain, and the transcription factor GATA-1. BmEos are functionally competent: they undergo chemotaxis toward mouse eotaxin-1 and produce characteristic cytokines, including IFN-gamma, IL-4, MIP-1alpha, and IL-6. The rodent pathogen pneumonia virus of mice replicates in bmEos and elevated levels of IL-6 are detected in supernatants of bmEos cultures in response to active infection. Finally, differentiating bmEos are readily transfected with lentiviral vectors, suggesting a means for rapid production of genetically manipulated cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.