ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2008
Retinal ON bipolar cells express a new PCP2 splice variant that accelerates the light response.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
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- Processing reliant on granule cells is essential for motor learning but dispensable for social preference and numerous other cerebellar-dependent behaviors.Nature communications · 2025Article
- Stimulating the regenerative capacity of the human retina with proneural transcription factors in 3D cultures.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A metabolome atlas of the aging mouse brain.Nature communications · 2021Article
- Arginyltransferase (Ate1) regulates the RGS7 protein level and the sensitivity of light-evoked ON-bipolar responses.Scientific reports · 2021Article
- A universal protocol for isolating retinal ON bipolar cells across species via fluorescence-activated cell sorting.Molecular therapy. Methods & clinical development · 2021Article
- The Transduction Cascade in Retinal ON-Bipolar Cells: Signal Processing and Disease.Annual review of vision science · 2017Review
- Minimal Purkinje Cell-Specific PCP2/L7 Promoter Virally Available for Rodents and Non-human Primates.Molecular therapy. Methods & clinical development · 2017Article
- Genetic Targeting in Cerebellar Purkinje Cells: an Update.Cerebellum (London, England) · 2017Review
- Lack of mGluR6-related cascade elements leads to retrograde trans-synaptic effects on rod photoreceptor synapses via matrix-associated proteins.The European journal of neuroscience · 2016Article
- Retinal Circuitry Balances Contrast Tuning of Excitation and Inhibition to Enable Reliable Computation of Direction Selectivity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- The TRPM1 channel in ON-bipolar cells is gated by both the α and the βγ subunits of the G-protein Go.Scientific reports · 2016Article
- Differential function of Gγ13 in rod bipolar and ON cone bipolar cells.The Journal of physiology · 2015Article
- Developmental upregulation of an alternative form of pcp2 with reduced GDI activity.Cerebellum (London, England) · 2014Article
- Two-photon imaging of nonlinear glutamate release dynamics at bipolar cell synapses in the mouse retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Sex-dependent behavioral functions of the Purkinje cell-specific Gαi/o binding protein, Pcp2(L7).Cerebellum (London, England) · 2012Article
- "mGlu Receptors in the Retina" - WIREs Membrane Transport and Signaling.Wiley interdisciplinary reviews. Membrane transport and signaling · 2012Article
- Gβ3 is required for normal light ON responses and synaptic maintenance.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- mGluR6 deletion renders the TRPM1 channel in retina inactive.Journal of neurophysiology · 2012Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
PCP2, a member of the GoLoco domain-containing family, is present exclusively in cerebellar Purkinje cells and retinal ON bipolar cells. Its function in these tissues is unknown. Biochemical and expression system studies suggest that PCP2 is a guanine nucleotide dissociation inhibitor, although a guanine nucleotide exchange factor has also been suggested. Here, we studied the function of PCP2 in ON bipolar cells because their light response depends on Galpha(o1), which is known to interact with PCP2. We identified a new splice variant of PCP2 (Ret-PCP2) and localized it to rod bipolar and ON cone bipolar cells. Electroretinogram recordings from PCP2-null mice showed a normal a-wave but a slower falling phase of the b-wave (generated by the activity of ON bipolar cells) relative to the wild type. Whole-cell recordings from rod bipolar cells showed, both under Ames medium and after blocking GABA(A/C) and glycine receptors, that PCP2-null rod bipolar cells were more depolarized than wild-type cells with greater inward current when clamped to -60 mV. Also under both conditions, the rise time of the response to intense light was slower by 28% (Ames) and 44% (inhibitory blockers) in the null cells. Under Ames medium, we also observed >30% longer decay time in the PCP2-null rod bipolar cells. We conclude that PCP2 facilitates cation channels closure in the dark, shortens the rise time of the light response directly, and accelerates the decay time indirectly via the inhibitory network. These data can most easily be explained if PCP2 serves as a guanine nucleotide exchange factor.
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