ArticleThe Journal of biological chemistry2008
Regulation of cyst wall protein promoters by Myb2 in Giardia lamblia.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Functionally-derivatized Nanoantibodies as novel tools for Giardia lamblia cyst detection.PLoS neglected tropical diseases · 2026Article
- Article
- A myeloid leukemia factor homolog is involved in tolerance to stresses and stress-induced protein metabolism in Giardia lamblia.Biology direct · 2023Article
- Identification of target genes regulated by encystation-induced transcription factor Myb2 using knockout mutagenesis in Giardia lamblia.Parasites & vectors · 2022Article
- A cell-cycle-dependent GARP-like transcriptional repressor regulates the initiation of differentiation inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Novel Spo11 Homologue Functions as a Positive Regulator in Cyst Differentiation inInternational journal of molecular sciences · 2021Article
- A Novel Multiprotein Bridging Factor 1-Like Protein Induces Cyst Wall Protein Gene Expression and Cyst Differentiation inInternational journal of molecular sciences · 2021Article
- DNA topoisomerase IIIβ promotes cyst generation by inducing cyst wall protein gene expression inOpen biology · 2020Article
- Article
- Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia.Nature communications · 2016Article
- Development of RNA Interference Trigger-Mediated Gene Silencing in Entamoeba invadens.Infection and immunity · 2016Article
- Time-Dependent Transcriptional Changes in Axenic Giardia duodenalis Trophozoites.PLoS neglected tropical diseases · 2015Article
- Identification of Giardia lamblia DHHC proteins and the role of protein S-palmitoylation in the encystation process.PLoS neglected tropical diseases · 2014Article
- DNA topoisomerase II is involved in regulation of cyst wall protein genes and differentiation in Giardia lamblia.PLoS neglected tropical diseases · 2013Article
- Regulation of a Myb transcription factor by cyclin-dependent kinase 2 in Giardia lamblia.The Journal of biological chemistry · 2012Article
- Functional redundancy of two Pax-like proteins in transcriptional activation of cyst wall protein genes in Giardia lamblia.PloS one · 2012Article
- A novel E2F-like protein involved in transcriptional activation of cyst wall protein genes in Giardia lamblia.The Journal of biological chemistry · 2011Article
- A novel pax-like protein involved in transcriptional activation of cyst wall protein genes in Giardia lamblia.The Journal of biological chemistry · 2010Article
- The transcriptional response to encystation stimuli in Giardia lamblia is restricted to a small set of genes.Eukaryotic cell · 2010Article
- Behind the smile: cell biology and disease mechanisms of Giardia species.Nature reviews. Microbiology · 2010Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myb family transcription factors are important in regulating cell proliferation, differentiation, and cell cycle progression. Giardia lamblia differentiates into infectious cysts to survive outside of the host. During encystation, genes encoding cyst wall proteins (CWPs) are coordinately induced. We have identified an encystation-induced Myb2 protein, which binds to the promoter regions of the cwp genes and myb2 itself in vitro. To elucidate the role of Myb2 in G. lamblia, we tested the hypothesis that Myb2 can activate encystation-induced genes. We found that overexpression of Myb2 resulted in an increase of expression of CWP1 at both protein and mRNA levels. Interestingly, the Myb2-overexpressing trophozoites had increased capability to differentiate into cysts. In cotransfection assays, Myb2 was able to transactivate the cwp promoters and its own promoter in vivo, suggesting that its gene can be positively autoregulated. Moreover, deletion of the N- or C-terminal domain resulted in a decrease of transactivation and autoregulation function of Myb2. We also found that the promoter of a newly identified encystation-induced gene, the giardial myeloid leukemia factor-like gene, has the Myb2 binding sites and that its mRNA levels were increased by Myb2 overexpression. Chromatin immunoprecipitation assays confirmed that Myb2 was bound to the promoters with its binding sites. Transfection of the myb2 antisense construct reduced the levels of the cwp1 transcripts and cyst formation. Our results suggest that Myb2 is a potent transactivator of the cwp genes and other endogenous genes and plays an important role in G. lamblia differentiation into cysts.
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