ArticleBiomaterials2008
Engineered extracellular matrices with cleavable crosslinkers for cell expansion and easy cell recovery.
Article in Biomaterials, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 104 citations in OpenAlex.
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- Highlighting the Influence between Physical and Chemical Cross-Linking in Dynamic Hydrogels for Two-Photon Micropatterning.Biomacromolecules · 2025Article
- Additive Manufacturing of Biomaterials-Design Principles and Their Implementation.Materials (Basel, Switzerland) · 2022Review
- A disulfide-based linker for thiol-norbornene conjugation: formation and cleavage of hydrogels by the use of light.Polymer chemistry · 2022Article
- A Redox-Based Autoinduction Strategy to Facilitate Expression of 5xCys-Tagged Proteins for Electrobiofabrication.Frontiers in microbiology · 2021Article
- Synthesis and characterization of a hyaluronic acid-based hydrogel with antioxidative and thermosensitive properties.RSC advances · 2020Article
- Multi-Domain Photopatterned 3D Tumor Constructs in a Micro-Physiological System for Analysis, Quantification, and Isolation of Infiltrating Cells.Advanced biosystems · 2020Article
- A multi-site metastasis-on-a-chip microphysiological system for assessing metastatic preference of cancer cells.Biotechnology and bioengineering · 2019Article
- 3D models of the bone marrow in health and disease: yesterday, today and tomorrow.MRS communications · 2019Article
- Injectable Biomaterials in Plastic and Reconstructive Surgery: A Review of the Current Status.Tissue engineering and regenerative medicine · 2018Review
- Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment.Chemical reviews · 2017Review
- A tunable hydrogel system for long-term release of cell-secreted cytokines and bioprinted in situ wound cell delivery.Journal of biomedical materials research. Part B, Applied biomaterials · 2017Article
- Exploration of Dynamic Elastic Modulus Changes on Glioblastoma Cell Populations with Aberrant EGFR Expression as a Potential Therapeutic Intervention Using a Tunable Hyaluronic Acid Hydrogel Platform.Gels (Basel, Switzerland) · 2017Article
- On-demand dissolution of modular, synthetic extracellular matrix reveals local epithelial-stromal communication networks.Biomaterials · 2017Article
- Therapeutic Restoration of Endothelial Glycocalyx in Sepsis.The Journal of pharmacology and experimental therapeutics · 2017Article
- Glycosaminoglycan-Based Biohybrid Hydrogels: A Sweet and Smart Choice for Multifunctional Biomaterials.Advanced materials (Deerfield Beach, Fla.) · 2016Review
- A reductionist metastasis-on-a-chip platform for in vitro tumor progression modeling and drug screening.Biotechnology and bioengineering · 2016Article
- Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling.Drug discovery today · 2016Review
- Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
An unmet need for expansion of primary cells and progenitor cells in three dimensions (3-D) is a synthetic mimic of the extracellular matrix (ECM) with user-controllable composition that would permit rapid recovery of viable cells under mild, non-enzymatic conditions. Three block copolymers based on disulfide-containing polyethylene glycol diacrylate crosslinkers were synthesized, and were used to crosslink thiol-modified hyaluronan and gelatin macromonomers in the presence of cells. The triblock PEGSSDA contained a single disulfide-containing block, the pentablock PEG(SS)(2)DA contained two disulfide blocks, and the heptablock PEG(SS)(3)DA contained three disulfide blocks. For each hydrogel composition, four cell types were encapsulated in 3-D, and growth and proliferation were evaluated. Murine NIH 3T3 fibroblasts, human HepG2 C3A hepatocytes, human bone marrow-derived mesenchymal stem cells (MSCs), and human umbilical vein endothelial cells (HUVECs) all showed excellent viability and growth during expansion in 3-D in the three disulfide block copolymer crosslinkers. After cell expansion, the hydrogels were dissociated using the thiol-disulfide exchange reaction in the presence of N-acetyl-cysteine or glutathione, which dissolved the hydrogel network. After dissolution, cells were recovered in high yield and with high viability by gentle centrifugation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.